CAJ | 학술논문

目的：探讨组织薄片法在人原代肝癌细胞培养中的应用。方法分别运用组织薄片法和组织块法从人肝癌组织标本中分离培养原代肝癌细胞，分析两种方法培养成功率、细胞爬出时间、形态及GPC3的表达。结果薄片法培养成功率为66．67％，爬出细胞时间为（7．89±0．78） d，在培养第一代就能观察到形态规则的细胞且周围培养环境洁净；组织块法培养成功率为61．11％，爬出细胞时间为（7．56±0．73）d，在培养第二代能观察到形态规则的细胞。同时原代肝癌细胞和HepG2均能稳定表达GPC3。结论两种方法均能成功培养出原代肝癌细胞，但薄片法培养的细胞在形态及洁净程度上较组织块好，同时能稳定表达GPC3。
목적：탐토조직박편법재인원대간암세포배양중적응용。방법분별운용조직박편법화조직괴법종인간암조직표본중분리배양원대간암세포，분석량충방법배양성공솔、세포파출시간、형태급GPC3적표체。결과박편법배양성공솔위66．67％，파출세포시간위（7．89±0．78） d，재배양제일대취능관찰도형태규칙적세포차주위배양배경길정；조직괴법배양성공솔위61．11％，파출세포시간위（7．56±0．73）d，재배양제이대능관찰도형태규칙적세포。동시원대간암세포화HepG2균능은정표체GPC3。결론량충방법균능성공배양출원대간암세포，단박편법배양적세포재형태급길정정도상교조직괴호，동시능은정표체GPC3。
Objective To investigate the application of the primary culture of liver cancer cells with tissue slice method. Methods The primary liver cancer cells from human liver tissue were isolated by using tissue slice method and tissue pieces respectively, and the success rate of cultivation, the cell climbed-out time, cell morphology, and the expression of GPC3 of two methods were analyzed. Results The success rate of cultivation with the tissue slice method was 66.67%,the cell climbed-out time was (7.89 ±0.78)..d..In the second generation of culture cells can be observed with morphological rules. Meanwhile HepG2 cells and primary liver cancer stably expressed GPC3. .Conclusion The both methods can successfully cultured primary liver cancer cells ,.but it is better with tissue slice method to raise the liver cancer cells in the cell morphology and cleanliness and which can stably express GPC3.