CAJ | 학술논문

为研究大黄鱼（Larimichthys crocea）冰鲜杂鱼饲料中细菌的多样性，用细菌通用引物构建了细菌16S rRNA基因克隆文库。从文库中随机挑选125个克隆子进行限制性片段长度多态性（ RFLP）分析，得到82个不同的RFLP 带型。对部分代表性克隆子进行测序，得到23条有效序列。序列同源性分析和系统进化分析结果表明，大黄鱼冰鲜杂鱼饲料中细菌主要分为3大类群，其中γ-变形菌纲（γ-Proteobacteria）细菌占据明显地优势地位，约占所分析克隆子数的84.4％；其次是黄杆菌纲（Flavobacteria）细菌，约占10.9％；还有少量的梭杆菌纲（Fusobac-teria）细菌，约占4.7％。γ-变形菌纲细菌中以嗜冷杆菌（Psychrobacter sp.）最多，其次是发光杆菌（Photobacteri-um）。
위연구대황어（Larimichthys crocea）빙선잡어사료중세균적다양성，용세균통용인물구건료세균16S rRNA기인극륭문고。종문고중수궤도선125개극륭자진행한제성편단장도다태성（ RFLP）분석，득도82개불동적RFLP 대형。대부분대표성극륭자진행측서，득도23조유효서렬。서렬동원성분석화계통진화분석결과표명，대황어빙선잡어사료중세균주요분위3대류군，기중γ-변형균강（γ-Proteobacteria）세균점거명현지우세지위，약점소분석극륭자수적84.4％；기차시황간균강（Flavobacteria）세균，약점10.9％；환유소량적사간균강（Fusobac-teria）세균，약점4.7％。γ-변형균강세균중이기랭간균（Psychrobacter sp.）최다，기차시발광간균（Photobacteri-um）。
To examine the bacterial composition in the feed mash of large yellow croaker(Larimichthys crocea),we constructed a 16S rRNA gene clone library using universal primers for the domain bacteria. 125 clones randomly selected from the library were screened by restriction fragment length polymorphism(RFLP)analysis. These clones were divided into 82 different RFLP patterns. Some repre-sentative clones were sequenced and 23 sequences were obtained. Sequence homology and phylogenetic analysis reveal high bacterial diversity in large yellow croaker′s feed mash. The sequences were clustered into three major groups,in which γ-Proterbacteria were the most predominant group,accounting for about 84. 4% of the sampled clones;Flavobacteria were the second dominant group,account-ing for about 10. 9%. There were a small number of Fusobacteria,accounting for about 4. 7%. Psychrobacter sp. were the most abun-dant members within γ-Proterbacteria,and Photobacterium were the second abundant members.