CAJ | 학술논문

将奥尼罗非鱼（Oreochromis niloticus × O. areus）幼鱼暴露于不同质量浓度（0、2.5 mg·L -1、5 mg·L -1、10 mg ·L -1和20 mg·L -1）的氨氮溶液中，于第0、第12、第24、第48和第72小时测定其非特异性免疫相关指标。结果表明，试验幼鱼经氨氮胁迫后各试验组血清溶菌酶活力随着胁迫时间的延长下降显著（P ﹤0.05）。氨氮胁迫明显影响鱼肝脏总抗氧化能力（T-AOC）及相关抗氧化酶活力。与对照组相比，随胁迫时间增加各试验组肝脏 T-AOC 及总超氧化物歧化酶（T-SOD）活力均先下降后上升（P ﹤0.05）。过氧化氢酶（CAT）及谷胱甘肽过氧化物酶（GSH-Px）活力变化还与胁迫溶液浓度相关。低浓度组（2.5 mg·L -1和5 mg·L -1），CAT 活力在胁迫24 h 后显著下降（P ﹤0.05）；高浓度组（10 mg·L -1和20 mg·L -1），CAT 活力却出现先升高后降低的变化。除最高浓度组，各试验组 GSH-Px 活力表现为诱导效应（P ﹤0.05）。试验条件下氨氮胁迫对罗非鱼非特异性免疫产生明显影响，且随浓度增加及时间延长影响程度加大。
장오니라비어（Oreochromis niloticus × O. areus）유어폭로우불동질량농도（0、2.5 mg·L -1、5 mg·L -1、10 mg ·L -1화20 mg·L -1）적안담용액중，우제0、제12、제24、제48화제72소시측정기비특이성면역상관지표。결과표명，시험유어경안담협박후각시험조혈청용균매활력수착협박시간적연장하강현저（P ﹤0.05）。안담협박명현영향어간장총항양화능력（T-AOC）급상관항양화매활력。여대조조상비，수협박시간증가각시험조간장 T-AOC 급총초양화물기화매（T-SOD）활력균선하강후상승（P ﹤0.05）。과양화경매（CAT）급곡광감태과양화물매（GSH-Px）활력변화환여협박용액농도상관。저농도조（2.5 mg·L -1화5 mg·L -1），CAT 활력재협박24 h 후현저하강（P ﹤0.05）；고농도조（10 mg·L -1화20 mg·L -1），CAT 활력각출현선승고후강저적변화。제최고농도조，각시험조 GSH-Px 활력표현위유도효응（P ﹤0.05）。시험조건하안담협박대라비어비특이성면역산생명현영향，차수농도증가급시간연장영향정도가대。
The juveniles of tilapia(Oreochromis niloticus × O. Areus)were exposed to ammonia-N(0,2. 5 mg·L - 1 ,5 mg·L - 1 , 10 mg·L - 1 and 20 mg·L - 1 )for 0,12 h,24 h,48 h and 72 h to evaluate the effect of ammonia-N stress on their non-specific im-munity. Results show that the activity of serum lysozyme decreased significantly with extension of stress time(P ﹤ 0. 05). The total antioxidant capacity(T-AOC)and activity of antioxidase in liver were significantly affected. The activity of T-AOC and total superox-ide dismutase(T-SOD)of fish exposed to ammonia-N were initially decreasing then increasing( P ﹤ 0. 05). Activities of catalase (CAT)and glutathione peroxidase(GSH-Px)were correlated with concentrations of ammonia-N. Fish exposed to lower concentra-tions(2. 5 mg·L - 1 ,5 mg·L - 1 )showed decreasing CAT activity within 24 h(P ﹤ 0. 05),while those exposed to higher concentra-tions(10 mg·L - 1 ,20 mg·L - 1 )showed initially increasing then decreasing activity of CAT. Except for the highest concentration groups,fish exposed to ammonia-N showed induction activity of GSH-Px(P ﹤ 0. 05). Under the experimental conditions,non-spe-cific immunity of tilapia was affected by ammonia-N stress,and the impact was increasing with increasing concentration and exten-sion of time.