CAJ | 학술논문

目的：观察Cdc20AAA/＋APCmin/＋基因型小鼠胚胎成纤维细胞（MEFs）的生物学性状，探讨Cdc20基因突变对MEFs生长的影响及机制。方法制作Cdc20AAA/＋APCmin/＋基因型、Cdc20AAA/＋基因型、APCmin/＋基因型和野生型（ WT）小鼠胚胎成纤维细胞，绘制生长曲线、进行平板克隆形成实验，比较各种基因型MEFs生长特性的改变。各种基因型MEFs核型分析染色体个数，并检测有丝分裂姐妹染色单体分离情况及是否存在染色体不稳定。结果Cdc20AAA/＋APCmin/＋基因型MEFs生长速度快于APCmin/＋基因型（P＜0.01）。 Cdc20AAA/＋APCmin/＋基因型MEFs克隆形成能力明显增强。 Cdc20AAA/＋APCmin/＋MEFs 中可见姐妹染色单体的提前分离，且染色体数目异常要多于APCmin/＋MEFs，大多数为38对。结论 Cdc20AAA/＋基因突变促进APCmin/＋小鼠胚胎成纤维细胞生长及增殖，使其呈现肿瘤细胞的生长特性，其机制可能与染色体不稳定有关。
목적：관찰Cdc20AAA/＋APCmin/＋기인형소서배태성섬유세포（MEFs）적생물학성상，탐토Cdc20기인돌변대MEFs생장적영향급궤제。방법제작Cdc20AAA/＋APCmin/＋기인형、Cdc20AAA/＋기인형、APCmin/＋기인형화야생형（ WT）소서배태성섬유세포，회제생장곡선、진행평판극륭형성실험，비교각충기인형MEFs생장특성적개변。각충기인형MEFs핵형분석염색체개수，병검측유사분렬저매염색단체분리정황급시부존재염색체불은정。결과Cdc20AAA/＋APCmin/＋기인형MEFs생장속도쾌우APCmin/＋기인형（P＜0.01）。 Cdc20AAA/＋APCmin/＋기인형MEFs극륭형성능력명현증강。 Cdc20AAA/＋APCmin/＋MEFs 중가견저매염색단체적제전분리，차염색체수목이상요다우APCmin/＋MEFs，대다수위38대。결론 Cdc20AAA/＋기인돌변촉진APCmin/＋소서배태성섬유세포생장급증식，사기정현종류세포적생장특성，기궤제가능여염색체불은정유관。
Objective Investigation of biological characteristics of Cdc 20AAA/+APCmin/+ mouse embryonic fibroblast(MEFs) indicate the effect of Cdc20AAA/+on growth of mouse embryonic fibroblast and the possible mechanism . Methods MEFs of Cdc20AAA/+APCmin/+, Cdc20AAA/+, APCmin/+ and WT genotype were harvested from embryos for analysis.The growth characteristics of Cdc20AAA/+APCmin/+, Cdc20AAA/+,APCmin/+and WT mouse embryonic fibroblast were analyzed through growth curve analysis and foci formation assay .Separation of sister chromatid and the presence of aneuploid were detected by karyotype analysis .Results Cell proliferation assays showed that Cdc 20AAA/+APCmin/+cells grew at an accelerated rate compared with APC min/+MEFs(P<0.01).Foci formation assay showed that the clone forming ability was significantly increased .Cdc20AAA/+APCmin/+MEFs showed a significant increase in the frequency of aneuploid compared with WT MEFs , which had a karyotype of 38 and contained prematurely separated sister chromatids .Conclusion Cdc20 carrying a null allele (Cdc20AAA/+) may accelerate the growth and proliferation of APC min/+MEFs and present the growth characteristics of the tumor cells .The possible mechanism may be associated with chromosome instability .