CAJ | 학술논문

目的：探讨褪黑素（ MLT）通过褪黑素膜受体MT2抑制小鼠前胃癌（ MFC）细胞增殖及其与丝裂原活化蛋白激酶（ MAPKs）、磷脂酰肌醇-3激酶（ PI3K）-Akt信号通路的关系。方法应用siRNA技术沉默MT2表达，观察褪黑素对小鼠前胃癌细胞的抑制作用及ERK1/2、Akt的磷酸化的影响。结果1.siRNA介导的MT2沉默能明显拮抗褪黑素对胃癌细胞增殖的抑制作用；2．沉默MT2可部分阻断褪黑素抑制ERK1/2、Akt磷酸化的作用。结论褪黑素可通过MT2受体抑制ERK1/2、Akt的磷酸化从而抑制胃癌细胞增殖。
목적：탐토퇴흑소（ MLT）통과퇴흑소막수체MT2억제소서전위암（ MFC）세포증식급기여사렬원활화단백격매（ MAPKs）、린지선기순-3격매（ PI3K）-Akt신호통로적관계。방법응용siRNA기술침묵MT2표체，관찰퇴흑소대소서전위암세포적억제작용급ERK1/2、Akt적린산화적영향。결과1.siRNA개도적MT2침묵능명현길항퇴흑소대위암세포증식적억제작용；2．침묵MT2가부분조단퇴흑소억제ERK1/2、Akt린산화적작용。결론퇴흑소가통과MT2수체억제ERK1/2、Akt적린산화종이억제위암세포증식。
Objective To investigate the inhibitory effect of melatonin on the proliferation activity of murine foregastic carcinomac ( MFC) cells via melatonin membrane receptors MT 2 and its relationship with the signaling pathways of mitogen-activated protein kinases ( MAPKs), phosphatidylinositol 3-kinase ( PI3K)-Akt.Methods Using siRNA technology to silence MT2 expression, we examined the ability of melatonin to inhibit the proliferation activity of MFC cells and its influence on the phosphorylation of ERK 1/2 and Akt.Results We found two interesting effects of SiRNA-mediated silencing of MT2 expression.Firstly, it significantly antagonized the inhibitory effect of melatonin on the proliferation activity of MFC cells .Secondly , it partially blocked the inhibitory effect of melatonin on the phosphorylation of ERK 1/2 and Akt.Conclusion Our results suggest that melatonin can inhibit the phosphorylation of ERK 1/2 and Akt via MT2 receptors , thereby inhibiting the proliferation of gastric cancer cells .