CAJ | 학술논문

目的：建立食蟹猴1-甲基-4-苯基-1，2，3，6-四氢吡啶（MPTP）帕金森病系统性模型，探讨嗅球中多巴胺（DA）及多巴胺/cAMP调节磷蛋白（DARPP32）的表达情况。方法3只成年健康食蟹猴，静脉注射MPTP，建立帕金森病系统性模型，取出嗅球，切片，免疫组织化学染色DA和DARPP32，摄片并观察DA和DARPP32在食蟹猴嗅球中的分布及表达情况，采用Image Pro-Plus软件，半定量分析模型组和正常组之间DA和DARPP32的表达差异。结果食蟹猴嗅球中DA和DARPP32神经元集中分布于突触小球层， DA神经纤维分布于突触小球层，而DARPP32神经纤维分布于嗅球各层，以突触小球层（ GL）和外网状层（ EPL）最为密集。 MPTP损伤后，与正常对照组比较，DA和DARPP32神经元及神经纤维均减少，以DA神经元及神经纤维减少明显。结论食蟹猴嗅球中DA神经元和神经纤维分布于突触小球层。食蟹猴MPTP帕金森病系统性模型的嗅球DA能神经元和纤维明显减少，可能与帕金森病嗅觉障碍有关。
목적：건립식해후1-갑기-4-분기-1，2，3，6-사경필정（MPTP）파금삼병계통성모형，탐토후구중다파알（DA）급다파알/cAMP조절린단백（DARPP32）적표체정황。방법3지성년건강식해후，정맥주사MPTP，건립파금삼병계통성모형，취출후구，절편，면역조직화학염색DA화DARPP32，섭편병관찰DA화DARPP32재식해후후구중적분포급표체정황，채용Image Pro-Plus연건，반정량분석모형조화정상조지간DA화DARPP32적표체차이。결과식해후후구중DA화DARPP32신경원집중분포우돌촉소구층， DA신경섬유분포우돌촉소구층，이DARPP32신경섬유분포우후구각층，이돌촉소구층（ GL）화외망상층（ EPL）최위밀집。 MPTP손상후，여정상대조조비교，DA화DARPP32신경원급신경섬유균감소，이DA신경원급신경섬유감소명현。결론식해후후구중DA신경원화신경섬유분포우돌촉소구층。식해후MPTP파금삼병계통성모형적후구DA능신경원화섬유명현감소，가능여파금삼병후각장애유관。
Objective To explore the localization and expression of dopaminergic neurons in olfactory bulb of cynomolgus monkeys damaged by 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP).Methods Three adult cynomolgus monkeys were injected with MPTP to induce the damage of dopamine neurons ( MPTP group ) and three adult cynomolgus monkeys were as a control group .Immunohistochemical staining was performed to examine the localization and expression of dopaminergic neurons in the olfactory bulb in normal and MPTP group monkeys .The numbers of DA-positive and DARPP32-positive cells were counted and the average absorbance was measured in normal and MPTP group .Results DA and DARPP32 positive neurons were concentrated in the glomerular layer ( GL) of olfactory bulb.DA positive nerve fibers were distributed in the GL while DARPP 32 positive nerve fibers appeared in all layers , and most nerve fibers were in GL and external plexiform layers (EPL).After MPTP injury, compared with the normal control group , DA and DARPP32 positive neurons and nerve fibers decreased in MPTP group and DA neurons and nerve fibers decreased significantly . Conclusions DA neurons and nerve fibers are in the GL of cynomolgus monkey olfactory bulb .DA neurons and fibers are significantly reduced in the olfactory bulb of cynomolgus monkeys damaged by MPTP , which may be associated with the dysosmia in Parkinson ’ s disease .