CAJ | 학술논문

目的：探讨节制饮食对猫初级视皮层内抑制性神经递质γ-氨基丁酸（ GABA）和脑源性神经营养因子（ BDNF）表达的影响。方法 Nissl染色示初级视皮层分层并用于神经元计数；免疫组织化学方法标记6只猫GABA和BDNF免疫阳性神经元。切片于Olympus光学显微镜下观察摄片，用Image-Pro Express 6.0软件进行细胞计数和免疫阳性反应的吸光度值统计。结果与正常饮食对照猫相比，节制饮食组猫的初级视皮层神经元平均密度无显著变化， GABA 免疫阳性神经元密度显著增大，阳性反应的平均吸光度值显著升高； BDNF免疫阳性细胞的密度和阳性反应的平均吸光度值亦明显增大。结论节制饮食能显著增强猫视皮层内GABA和BDNF的表达，这可能对视皮层神经元的结构和功能有保护作用，从而延缓衰老进程。
목적：탐토절제음식대묘초급시피층내억제성신경체질γ-안기정산（ GABA）화뇌원성신경영양인자（ BDNF）표체적영향。방법 Nissl염색시초급시피층분층병용우신경원계수；면역조직화학방법표기6지묘GABA화BDNF면역양성신경원。절편우Olympus광학현미경하관찰섭편，용Image-Pro Express 6.0연건진행세포계수화면역양성반응적흡광도치통계。결과여정상음식대조묘상비，절제음식조묘적초급시피층신경원평균밀도무현저변화， GABA 면역양성신경원밀도현저증대，양성반응적평균흡광도치현저승고； BDNF면역양성세포적밀도화양성반응적평균흡광도치역명현증대。결론절제음식능현저증강묘시피층내GABA화BDNF적표체，저가능대시피층신경원적결구화공능유보호작용，종이연완쇠로진정。
Objective To explore the effect of DR on the expression of inhibitory neurotransmitter ,γ-aminobutyric acid ( GABA) and brain derived neurotrophic factor ( BDNF) in the primary visual cortex of young cats .Methods Totally 6 cats were wsed in this study . Nissl staining was used for cortical layer identification and cell counting . Immunohistochemical techniques were utilized to label GABA-and BDNF-positive neurons .Sections were observed under an Olympus light microscope and photographed with a digital camera .The cell density and absorbance of immunoreactivity were measured with Image-Pro Express 6.0 softwares .Results Our results showed that the mean density of Nissl-stained neurons in the primary visual cortex ( V1) of the DR group showed no significant difference from that of the control group . However , the mean density of GABA-immunoreactive neurons in each cortical layer of V 1 in DR cats was significantly higher than that in control cats .The mean immunoreactive intensity of GABA-positive neurons , as indicated by the average absorbance , increased significantly in DR cats relative to control ones . Accompanied with the elevation of GABA expression, DR enhanced BDNF expression in V1, as indicated by an increased mean density of BDNF-positive neurons and BDNF-immunoreactive average absorbance in DR cats relative to controls .Conclusion These results indicate that dietary restriction leads to a concurrent expression upregulation of GABA and BDNF , which may likely compensate for brain functional degradation during senescence and thus delay aging process .