解剖学报
解剖學報
해부학보
ACTA ANATOMICA SINICA
2014年
3期
310-315
,共6页
王婷婷%滕蕾%黄惠%李艳%孙丽
王婷婷%滕蕾%黃惠%李豔%孫麗
왕정정%등뢰%황혜%리염%손려
脑缺血%再灌注损伤%黄芪注射液%c-Jun氨基末端激酶3%凋亡%免疫印迹法%大鼠
腦缺血%再灌註損傷%黃芪註射液%c-Jun氨基末耑激酶3%凋亡%免疫印跡法%大鼠
뇌결혈%재관주손상%황기주사액%c-Jun안기말단격매3%조망%면역인적법%대서
Cerebral ischemia%Reperfusion injury%Astragalus injection%c-Jun N-terminal kinase 3%Apoptosis%Western blotting%Rat
目的:探讨黄芪注射液对大鼠脑缺血再灌注损伤的神经保护作用和机制。方法成年健康雄性Wistar大鼠70只,应用线栓法经左侧颈外-颈内动脉插线建立大脑中动脉阻塞再灌注模型,经腹腔注射1g/L黄芪注射液(3ml/kg)干预治疗。 Longa法评价大鼠神经功能缺损程度,氯化三苯基四氮唑( TTC)染色观察脑梗死体积,苏木素-伊红染色观察顶叶皮质区神经元形态结构变化,透射电子显微镜观察神经细胞的超微结构,流式细胞术检测细胞凋亡,Western blotting检测c-Jun氨基末端激酶3(JNK3)蛋白表达,RT-PCR检测JNK3 mRNA表达。结果经黄芪注射液治疗后,大鼠顶叶皮质区神经元JNK3 mRNA和JNK3蛋白表达较对照组显著减低,凋亡细胞数量显著减少,脑梗死体积显著缩小,神经元形态和超微结构以及动物神经行为功能显著改善。结论黄芪注射液可能通过下调神经元JNK3基因表达而抑制细胞凋亡,缩小脑梗死体积,改善动物的神经行为功能。
目的:探討黃芪註射液對大鼠腦缺血再灌註損傷的神經保護作用和機製。方法成年健康雄性Wistar大鼠70隻,應用線栓法經左側頸外-頸內動脈插線建立大腦中動脈阻塞再灌註模型,經腹腔註射1g/L黃芪註射液(3ml/kg)榦預治療。 Longa法評價大鼠神經功能缺損程度,氯化三苯基四氮唑( TTC)染色觀察腦梗死體積,囌木素-伊紅染色觀察頂葉皮質區神經元形態結構變化,透射電子顯微鏡觀察神經細胞的超微結構,流式細胞術檢測細胞凋亡,Western blotting檢測c-Jun氨基末耑激酶3(JNK3)蛋白錶達,RT-PCR檢測JNK3 mRNA錶達。結果經黃芪註射液治療後,大鼠頂葉皮質區神經元JNK3 mRNA和JNK3蛋白錶達較對照組顯著減低,凋亡細胞數量顯著減少,腦梗死體積顯著縮小,神經元形態和超微結構以及動物神經行為功能顯著改善。結論黃芪註射液可能通過下調神經元JNK3基因錶達而抑製細胞凋亡,縮小腦梗死體積,改善動物的神經行為功能。
목적:탐토황기주사액대대서뇌결혈재관주손상적신경보호작용화궤제。방법성년건강웅성Wistar대서70지,응용선전법경좌측경외-경내동맥삽선건립대뇌중동맥조새재관주모형,경복강주사1g/L황기주사액(3ml/kg)간예치료。 Longa법평개대서신경공능결손정도,록화삼분기사담서( TTC)염색관찰뇌경사체적,소목소-이홍염색관찰정협피질구신경원형태결구변화,투사전자현미경관찰신경세포적초미결구,류식세포술검측세포조망,Western blotting검측c-Jun안기말단격매3(JNK3)단백표체,RT-PCR검측JNK3 mRNA표체。결과경황기주사액치료후,대서정협피질구신경원JNK3 mRNA화JNK3단백표체교대조조현저감저,조망세포수량현저감소,뇌경사체적현저축소,신경원형태화초미결구이급동물신경행위공능현저개선。결론황기주사액가능통과하조신경원JNK3기인표체이억제세포조망,축소뇌경사체적,개선동물적신경행위공능。
Objective To investigate the neuroprotective effect and mechanism of astragalus injection in cerebral ischemia reperfusion injury in rats .Methods A total of 70 healthy adult male Wistar rats were subjected to establish middle cerebral artery occlusion reperfusion models by inserting a monofilament thread from the external -internal carotid artery and treated by injecting 1g/L intraperitoneally astragalus injection (3ml/kg).The neurobehavioral function of rats was evaluated by Longa ’ s test and the cerebral infarction volume was calculated by tetrazolium chloride staining .The shape and ultrastructure of neurons in parietal cortex were observed by HE stain TEM .The early apoptotic ratio of neurons was detected by flow cytometry .The expressions of c-Jun N-terminal kinase 3 ( JNK3) mRNA and protein were determined by RT-PCR and Western blotting , respectively.Results After treatment with astragalus injection , the expressions of JNK3 mRNA and protein reduced significantly , the number of neuronal apoptosis in parietal cortexminus , the cerebral infarction volume shrink, the neuronal shape and ultrastructure and animal neurobehavioral function were improved significantly than those in model group rats .Conclusion The results suggest that astragalus injection may inhibit neuronal apoptosis , reduce the infarction volume and improve the animal neurobehavioral function by down -regulated the expression of JNK 3 gene following cerebral ischemia in rats .