广西植物
廣西植物
엄서식물
GUIHAIA
2014年
3期
419-425
,共7页
考安都%王述贵%王艳芹%徐洲%周永红%丁春邦
攷安都%王述貴%王豔芹%徐洲%週永紅%丁春邦
고안도%왕술귀%왕염근%서주%주영홍%정춘방
油茶%遗传多样性%ISSR%RAPD%聚类分析
油茶%遺傳多樣性%ISSR%RAPD%聚類分析
유다%유전다양성%ISSR%RAPD%취류분석
Camellia oleifera%genetic diversity%ISSR%RAPD%cluster analysis
利用 ISSR 和 RAPD 标记,对名邛台地野生油茶种质进行遗传多样性分析.从60条简单重复序列引物中筛选出16条引物,在65份样品中共扩增出213条带,其中多态位点为203个,多态位点百分率为95.31％；从30条寡居核苷酸引物中筛选出8条引物,共扩增出105条带,其中多态性位点94个,多态位点百分率为89．52％.结果表明：名邛台地野生油茶种质具有较丰富的遗传多样性,ISSR 和 RAPD 标记可以应用于油茶种质遗传多样性分析.
利用 ISSR 和 RAPD 標記,對名邛檯地野生油茶種質進行遺傳多樣性分析.從60條簡單重複序列引物中篩選齣16條引物,在65份樣品中共擴增齣213條帶,其中多態位點為203箇,多態位點百分率為95.31％；從30條寡居覈苷痠引物中篩選齣8條引物,共擴增齣105條帶,其中多態性位點94箇,多態位點百分率為89．52％.結果錶明：名邛檯地野生油茶種質具有較豐富的遺傳多樣性,ISSR 和 RAPD 標記可以應用于油茶種質遺傳多樣性分析.
이용 ISSR 화 RAPD 표기,대명공태지야생유다충질진행유전다양성분석.종60조간단중복서렬인물중사선출16조인물,재65빈양품중공확증출213조대,기중다태위점위203개,다태위점백분솔위95.31％；종30조과거핵감산인물중사선출8조인물,공확증출105조대,기중다태성위점94개,다태위점백분솔위89．52％.결과표명：명공태지야생유다충질구유교봉부적유전다양성,ISSR 화 RAPD 표기가이응용우유다충질유전다양성분석.
The genetic diversity among 65 wild Camellia oleifera from Mingqiong platform were discussed.213 loci were identified with 16 simple sequence repeat primers screened from 60 primers,out of which 203 loci were poly-morphic and the percentage of polymorphic bands was 96.31%;105 loci were identified with 8 oligonucleotide primers screened from 30 primers,out of which 94 loci were polymorphic and the percentage of polymorphic bands was 89.52%.The results showed that ISSR and RAPD markers could be used in genetic diversity analysis,C .oleifera had a high level of genetic diversity.
