CAJ | 학술논문

采用 MTT 法检测细胞活力,用倒置显微镜、荧光显微镜和扫描电子显微镜观察细胞形态与结构的变化,用激光共聚焦显微镜观察细胞微管的分布,从而研究了野艾蒿挥发油对 HeLa 人宫颈癌细胞形态与结构的影响。结果表明：(1)野艾蒿挥发油对 HeLa 癌细胞的增殖有明显的抑制作用,呈剂量和时间依赖性。(2)野艾蒿挥发油处理 HeLa 癌细胞24 h 后,100、200μg/mL 实验组细胞体积缩小,核染色质凝集、微绒毛消失、细胞表面有泡状突起,微管解聚,呈现典型的凋亡特征；400μg/mL 实验组细胞膜破裂、胞浆内含物外泄,呈明显的坏死特征。(3)野艾蒿挥发油具有抑制 HeLa 癌细胞增殖的作用,低、中浓度的野艾蒿挥发油诱导细胞凋亡,而高浓度的野艾蒿挥发油引起细胞坏死。
채용 MTT 법검측세포활력,용도치현미경、형광현미경화소묘전자현미경관찰세포형태여결구적변화,용격광공취초현미경관찰세포미관적분포,종이연구료야애호휘발유대 HeLa 인궁경암세포형태여결구적영향。결과표명：(1)야애호휘발유대 HeLa 암세포적증식유명현적억제작용,정제량화시간의뢰성。(2)야애호휘발유처리 HeLa 암세포24 h 후,100、200μg/mL 실험조세포체적축소,핵염색질응집、미융모소실、세포표면유포상돌기,미관해취,정현전형적조망특정；400μg/mL 실험조세포막파렬、포장내함물외설,정명현적배사특정。(3)야애호휘발유구유억제 HeLa 암세포증식적작용,저、중농도적야애호휘발유유도세포조망,이고농도적야애호휘발유인기세포배사。
The effects of essential oil from Artemisia lavandulaefolia on morphology and structure of HeLa cells were investigated.Cell viability was assayed using MTT method.The differences of cell morphology and structure were observed by inverted microscopy,fluorescence and scanning electron microscope.Furthermore, the distribution of microtubules was observed by confocal microscopy.The results showed that essential oil from Artemisia lavandulaefolia could inhibit the proliferation of HeLa cells in dose-and time-dependent man-ner.Treated with essential oil of Artemisia lavadulaefolia for 24 h,cells in 100 and 200 μg??mL-1 experiment groups exhibited the typical morphology changes of undergoing apoptosis,such as cell shrinkage,nucleus chro-matin condensed,microvilli disappear,bubbly uplift and the microtubules depolymerized.However,the cells in the 400 μg??mL-1 group showed the necrotic morphology changes including cytomembrane rupture and cyto-plasm spillover.Essential oil from Artemisia lavadulaefolia could inhibit the proliferation of HeLa cells in vitro .And low and middle concentrations of essential oil from Artemisia lavadulaefolia could induce apopto-sis,whereas high concentration of the compounds resulted in necrosis in HeLa cells.