CAJ | 학술논문

以马来沉香茎段为外植体,分别对外植体的消毒、启动培养、增殖培养、壮苗培养、生根培养、炼苗移栽环节进行研究,着重探索马来沉香组织培养技术各个环节的最佳培养基配方,为马来沉香的工厂化育苗提供技术指导.结果表明：马来沉香最佳消毒方法是用0．1％升汞消毒4~5 min；启动率最高的培养基配方是1/2 MS＋6-BA 0．2 mg??L-1＋NAA 0．1 mg??L-1＋蔗糖30 g??L-1＋琼脂5．8 g??L-1,启动率达70．5％；增殖系数最高的培养基是1/2 MS＋0．1 mg??L-16-BA＋25 g??L-1蔗糖＋5．8 g??L-1琼脂,增殖系数达2．9；最佳壮苗培养基是1/2 MS＋30 g??L-1蔗糖＋5．8 g??L-1琼脂；最佳生根培养基为1/2 MS＋NAA 5．0 mg??L-1＋20 g??L-1糖＋6 g??L-1琼脂,培养2 d 后移入1/2 MS 培养基继续培养,生根率为83％；马来沉香移栽较难成活,在泥炭土∶黄泥土(2∶1)的基质上成活率最高,移栽成活率65％.
이마래침향경단위외식체,분별대외식체적소독、계동배양、증식배양、장묘배양、생근배양、련묘이재배절진행연구,착중탐색마래침향조직배양기술각개배절적최가배양기배방,위마래침향적공엄화육묘제공기술지도.결과표명：마래침향최가소독방법시용0．1％승홍소독4~5 min；계동솔최고적배양기배방시1/2 MS＋6-BA 0．2 mg??L-1＋NAA 0．1 mg??L-1＋자당30 g??L-1＋경지5．8 g??L-1,계동솔체70．5％；증식계수최고적배양기시1/2 MS＋0．1 mg??L-16-BA＋25 g??L-1자당＋5．8 g??L-1경지,증식계수체2．9；최가장묘배양기시1/2 MS＋30 g??L-1자당＋5．8 g??L-1경지；최가생근배양기위1/2 MS＋NAA 5．0 mg??L-1＋20 g??L-1당＋6 g??L-1경지,배양2 d 후이입1/2 MS 배양기계속배양,생근솔위83％；마래침향이재교난성활,재니탄토∶황니토(2∶1)적기질상성활솔최고,이재성활솔65％.
Using the young shoots as explants,the methods including sterilization,induction,propagation,rooting and planting of the Aquilaria malaccensis were studied.It was indicated that the best method to sterilize was 4-5 min as 0.1% HgCl2 ;1/2 MS+6-BA 0.2 mg??L-1 +NAA 0.1 mg??L-1 +sugar 30 g??L-1 + agar 5.8 g??L-1 as the ef-fective medium for adventitious shoot induction,the induction rate was 70.5%;1/2 MS+0.1 mg??L-1 6-BA+sugar 25 g??L-1 +agar 5.8 g??L-1 as the suitable propagation medium,and the coefficient was 2.9;the medium of 1/2 MS+ sugar 30 g??L-1 + agar 5.8 g??L-1 made the emblings grow strong to cut;1/2 MS+NAA 5.0+sugar 20 g??L-1+agar 6.0 g??L-1 as the rooting medium,the rooting plantlets would be transferred to the medium with no hormone after two days.The rooting rate was 83%.It was a little difficult to transplant,and the survival rate was only 65%in the medium mixed with peat soil and yellow mud (proportion was 2∶1).