CAJ | 학술논문

分析了青天葵及其混伪品 matK 序列,以期在分子水平建立青天葵及其常见混伪品的鉴别方法.采用一对通用引物对 matK 基因进行 PCR 扩增并测序,所得序列用 DNAMAN、MEGA 等软件进行分析.获得青天葵及其混伪品的 matK 基因序列长度为587 bp,平均 GC 含量为32．8％.青天葵的种内遗传距离为0,与混伪品种间遗传距离范围为0．016~0．375.基于 matK 基因序列构建的 NJ 聚类树能明显地区别青天葵及其混伪品.因此,应用 matK 基因序列可以有效鉴别青天葵及其混伪品.
분석료청천규급기혼위품 matK 서렬,이기재분자수평건립청천규급기상견혼위품적감별방법.채용일대통용인물대 matK 기인진행 PCR 확증병측서,소득서렬용 DNAMAN、MEGA 등연건진행분석.획득청천규급기혼위품적 matK 기인서렬장도위587 bp,평균 GC 함량위32．8％.청천규적충내유전거리위0,여혼위품충간유전거리범위위0．016~0．375.기우 matK 기인서렬구건적 NJ 취류수능명현지구별청천규급기혼위품.인차,응용 matK 기인서렬가이유효감별청천규급기혼위품.
In order to establish the novel method on distinguishing Nervilia fordii and its common adulterants,matK genes from N .fordii and its adulterants were amplified and sequenced using a pair of universal primes.The results revealed that matK genes acquired from N .fordii and its adulterants contained 587 base pairs,and average GC con-tent was 32.8%.The intraspecific distances among N .fordii were 0,whereas the interspecific distance between N . fordii and its adulterants ranged from 0.016 to 0.375.The clustering tree according to Neighbor Joining method and Kimura 2-Parameter model discriminated N .fordii and its adulterants obviously.matK gene is suggested to be an effective biomarker to identify N .fordii and its common adulterants.