CAJ | 학술논문

探讨五味子中残留农药对卵巢颗粒细胞的毒性作用．MTT 法测定农药提取物作用于卵巢颗粒细胞24、48、72 h后的细胞增殖情况；Western blot法检测农药提取物对卵巢颗粒细胞MAPK信号通路的ERK1/2及p-ERK1/2蛋白表达水平的影响．MTT结果显示农药提取物能够显著促进卵巢颗粒细胞增殖，48 h增殖率最高（P＜0．01），Western blot 结果显示提取物能够促进ERK1/2、p-ERK1/2蛋白表达，与阴性对照组相比差异极显著（ P＜0．01）．因此农药提取物作用于体外培养的卵巢颗粒细胞能够显著促进细胞增殖，通过促进MAPK信号通路的ERK1/2、p-ERK1/2蛋白表达，对颗粒细胞产生毒性作用．
탐토오미자중잔류농약대란소과립세포적독성작용．MTT 법측정농약제취물작용우란소과립세포24、48、72 h후적세포증식정황；Western blot법검측농약제취물대란소과립세포MAPK신호통로적ERK1/2급p-ERK1/2단백표체수평적영향．MTT결과현시농약제취물능구현저촉진란소과립세포증식，48 h증식솔최고（P＜0．01），Western blot 결과현시제취물능구촉진ERK1/2、p-ERK1/2단백표체，여음성대조조상비차이겁현저（ P＜0．01）．인차농약제취물작용우체외배양적란소과립세포능구현저촉진세포증식，통과촉진MAPK신호통로적ERK1/2、p-ERK1/2단백표체，대과립세포산생독성작용．
The research method of the pesticide residues toxic effect from schisandra on the o-varian granulose cell was explored .MTT assay was used to determine pesticide extract on o-varian granulosa cells after 24, 48,72 h cell proliferation,and Western blot was used to de-tect the impact of pesticide extract on the expression levels of ERK 1/2 and p-ERK1/2 pro-tein of MAPK signal pathway of ovarian granulosa cell .MTT results showed that pesticide ex-tract could significantly promote cell proliferation , and the highest growth rate was at 48 h ( P <0.01 ) .Western blot showed that pesticide extract could promote the expression of ERK1/2 and p-ERK1/2 protein , which compared with the negative control group had sig-nificant difference ( P <0 .01 ) .Therefore pesticides extract on ovarian granulosa cells in vitro could significantly promote cell proliferation by promoting the expression of ERK 1/2 , p-ERK1/2 protein of MAPK signal pathway to produce toxic effects for granulosa cells .