CAJ | 학술논문

目的：研究低强度超声结合5-氨基酮戊酸（5-ALA）对大鼠骨肉瘤UMR-106细胞的杀伤机制。方法将处于对数生长期的大鼠骨肉瘤UMR-106细胞分成对照组、5-ALA组、超声组和超声＋5-氨基酮戊酸组（SDT组）。流式细胞仪检测细胞凋亡率，活性氧（ROS）的产生，线粒体膜电位（MMP）的改变；荧光显微镜观察33342染色细胞核的形态改变；透射电镜观察超微结构改变。结果当超声频率为1．0MHz，声强2．0W/cm2，5-ALA浓度2mmol/L，SDT组与对照组、超声组及5-ALA组比较，其凋亡率（32．2±1．4）％，明显增高（P＜0．05）。同时伴有ROS（34．4±2．4）％的产生和MMP（42．2±2．6）％的降低。通过33342染色荧光显微镜观察到超声联合5-ALA组的细胞核发生浓缩和碎裂。透射电镜观察到细胞膜、线粒体、高尔基体等细胞器改变及凋亡小体的形成。结论低强度超声联合5-ALA对UMR-106细胞的杀伤作用明显，细胞以凋亡为主，其线粒体途径对UMR-106细胞凋亡起着重要作用。
목적：연구저강도초성결합5-안기동무산（5-ALA）대대서골육류UMR-106세포적살상궤제。방법장처우대수생장기적대서골육류UMR-106세포분성대조조、5-ALA조、초성조화초성＋5-안기동무산조（SDT조）。류식세포의검측세포조망솔，활성양（ROS）적산생，선립체막전위（MMP）적개변；형광현미경관찰33342염색세포핵적형태개변；투사전경관찰초미결구개변。결과당초성빈솔위1．0MHz，성강2．0W/cm2，5-ALA농도2mmol/L，SDT조여대조조、초성조급5-ALA조비교，기조망솔（32．2±1．4）％，명현증고（P＜0．05）。동시반유ROS（34．4±2．4）％적산생화MMP（42．2±2．6）％적강저。통과33342염색형광현미경관찰도초성연합5-ALA조적세포핵발생농축화쇄렬。투사전경관찰도세포막、선립체、고이기체등세포기개변급조망소체적형성。결론저강도초성연합5-ALA대UMR-106세포적살상작용명현，세포이조망위주，기선립체도경대UMR-106세포조망기착중요작용。
Objective To investigate the killing effect of low -intensity ultrasound combined with 5-aminolevulinic acid (5-ALA) on rat osteosarcoma cell line UMR -106.Methods After the UMR-106 cells came into logarithm growth period , they were divided into control group and experimental group .Cell apoptotic rate ,the production of reactive oxygen species (ROS) and the change of mitochondrial membrane potential ( MMP) was observed by flow cytometry;ultrastructural changes were observed by transmission electron microscope ( TEM) .Results Using low intensity ultrasound of 1.0 MHz and 2.0 W/cm2 , plus 5-ALA at a concentration of 2 mmol/L.The apoptotic rate of SDT group was (32.2 ±1.4)%which was signifi-cantly higher than that of control group, ultrasound group and 5 -ALA group(P <0.05).The production of ROS was (34.4 ±2.4)%and the decrease of MMP was (42.2 ±2.6)%.Through the 33342 staining was observed in ultrasound com-bined with 5-ALA group of nuclear condensation and fragmentation .The structural changes of cell membrane, mitochondria Golgi apparatus and other organelles could be observed by TEM clearly Formation of apoptotic bodies .Conclu sion The killing effect of low-intensity ultrasound combined with 5-ALA on UMR-106 cells was significantly .Cell apoptosis played a vital role in the killing effect, and the mitochondria pathway contributed mainly in the apoptosis of UMR -106 cells.