分析仪器
分析儀器
분석의기
ANALYTICAL INSTRUMENTATION
2014年
3期
50-55
,共6页
高一龙%温海%宋珍华%吴德华%马大君%秦海斌
高一龍%溫海%宋珍華%吳德華%馬大君%秦海斌
고일룡%온해%송진화%오덕화%마대군%진해빈
犬%STR基因座%遗传多态性%荧光标记%个体识别和亲权鉴定
犬%STR基因座%遺傳多態性%熒光標記%箇體識彆和親權鑒定
견%STR기인좌%유전다태성%형광표기%개체식별화친권감정
dog%STR loci%genetic polymorphisms%fluorescent-labeled%individual discrimination and paternity identification
选择16个STR基因座，应用五色荧光标记技术，使用ABI3130基因分析仪对80头拉布拉多犬的遗传多态性进行检测分析。研究表明：这16个S T R基因座建立的荧光标记复合扩增体系，各个基因座扩增平衡，结果均一稳定；16个STR 基因座共检测到164个等位基因，平均杂合度（H）和平均多态信息含量（ PIC）分别为0.881和0.813；而累积非父排除率（CPE ）和累积个体识别率（ TDP ）分别为0.999999999997和0.9999999999999999。这16个S T R基因座建立的荧光标记复合扩增体系，可以有效用于犬的个体识别和亲权鉴定。
選擇16箇STR基因座，應用五色熒光標記技術，使用ABI3130基因分析儀對80頭拉佈拉多犬的遺傳多態性進行檢測分析。研究錶明：這16箇S T R基因座建立的熒光標記複閤擴增體繫，各箇基因座擴增平衡，結果均一穩定；16箇STR 基因座共檢測到164箇等位基因，平均雜閤度（H）和平均多態信息含量（ PIC）分彆為0.881和0.813；而纍積非父排除率（CPE ）和纍積箇體識彆率（ TDP ）分彆為0.999999999997和0.9999999999999999。這16箇S T R基因座建立的熒光標記複閤擴增體繫，可以有效用于犬的箇體識彆和親權鑒定。
선택16개STR기인좌，응용오색형광표기기술，사용ABI3130기인분석의대80두랍포랍다견적유전다태성진행검측분석。연구표명：저16개S T R기인좌건립적형광표기복합확증체계，각개기인좌확증평형，결과균일은정；16개STR 기인좌공검측도164개등위기인，평균잡합도（H）화평균다태신식함량（ PIC）분별위0.881화0.813；이루적비부배제솔（CPE ）화루적개체식별솔（ TDP ）분별위0.999999999997화0.9999999999999999。저16개S T R기인좌건립적형광표기복합확증체계，가이유효용우견적개체식별화친권감정。
We tried to construct a five-fluorescent-labeled multiplex amplification system including 16 STR loci ,analyze the genetic polymorphisms of 80 Labrador Retrievers by ABI3130 genetic analyzer .The results showed the 16 STR loci multiplex system was constructed successfully ,with good amplification product ,good intracolor and intercolor balance .164 alleles were detected in 16 STR loci ,the average PIC and heterozygosity were 0 .881 and 0 .813 respeclively .The cumulative probability of paternity exclusion (CPE) and total power of discrimination (TDP) were 0 .999999999997 and 0 .9999999999999999 respec-tively .The five-fluorescent-labeled 16 STR loci multiplex system had been successfully constructed ,could be applied to canine individual discrimination and paternity identification .
