CAJ | 학술논문

本文采用高效液相色谱法，以乙腈-水（50：50，v/v）混合溶液为流动相，经 YMC -C18（250 mm ×46mm ，5μm）色谱柱分离，紫外检测波长为270nm时，测定了苯、苯酚、对苯醌、邻苯二酚、间苯二酚、对苯二酚和苯三酚。结果表明：苯、苯酚、对苯醌、邻苯二酚、间苯二酚、对苯二酚和苯三酚在各自的测定范围内均有良好的线性关系（相关系数为≥0.999），方法的检出限为0.002～0.008μg/mL。内标法（ISM ）回收率为80.40％～101.59％，相对标准偏差＜9.6％；外标法（ESM ）回收率为82.72％～99.48％，相对标准偏差＜10.8％。说明在苯、苯酚、对苯醌、邻苯二酚、间苯二酚、对苯二酚和苯三酚定量分析方法中内标法优于外标法，并且该方法操作简便、快速、稳定性好、样品和溶液用量少，分析条件温和。
본문채용고효액상색보법，이을정-수（50：50，v/v）혼합용액위류동상，경 YMC -C18（250 mm ×46mm ，5μm）색보주분리，자외검측파장위270nm시，측정료분、분분、대분곤、린분이분、간분이분、대분이분화분삼분。결과표명：분、분분、대분곤、린분이분、간분이분、대분이분화분삼분재각자적측정범위내균유량호적선성관계（상관계수위≥0.999），방법적검출한위0.002～0.008μg/mL。내표법（ISM ）회수솔위80.40％～101.59％，상대표준편차＜9.6％；외표법（ESM ）회수솔위82.72％～99.48％，상대표준편차＜10.8％。설명재분、분분、대분곤、린분이분、간분이분、대분이분화분삼분정량분석방법중내표법우우외표법，병차해방법조작간편、쾌속、은정성호、양품화용액용량소，분석조건온화。
Based on the high performance liquid chromatography method ,benzene and its direct oxida-tion products :phenol , benzoquinone , o-Bihydroxybenzene , m-Bihydroxybenzene , p-Bihydroxybenzene , benzenetriol were completely separated by the YMC-C18(250mm × 46mm ,5μm) chromatographic column with acetonitrile-water(50:50 ,v/v) as mobile phase .The UV detection wavelength was set at 270 nm .The result showed good linearity (R≥0 .99) ,the detection limit of the samples were between 0 .002-0 .008 μg/mL .The recoveries of internal standard method(ISM) ranged from 80 .40 to 101 .59% at the three spiked levels ,the relative standard deviation was below 9 .6% ;the recoveries of external standard method (ESM ) ranged from 82 .72 to 99 .48% at three spiked levels ,the relative standard deviation was below 10 .8% .It was indicated that the internal standard method was better than the external standard method for the quan-titative analysis of benzene and its products of oxidation .T his method is simple ,rapid ,sensitive and stabi-lized ,low consumption of sample and solution ,and analytical conditions are moderate .