长治医学院学报
長治醫學院學報
장치의학원학보
JOURNAL OF CHANGZHI MEDICAL COLLEGE
2014年
3期
190-193
,共4页
韩学波%唐秀英%李莉%于欣
韓學波%唐秀英%李莉%于訢
한학파%당수영%리리%우흔
全自动生化分析仪%电解质项目%性能验证
全自動生化分析儀%電解質項目%性能驗證
전자동생화분석의%전해질항목%성능험증
automatic biochemical Analyzer%electrolyte proj ects%performance verification
目的：评价奥林巴斯 AU2700全自动生化分析仪测定电解质项目的分析性能。方法：按照美国临床实验室标准化组织(CLSI)指南文件和其他相关文献的实验方案,分析奥林巴斯 AU2700全自动生化分析仪测定电解质项目的精密度、准确度、线性及可报告范围等。结果：AU2700全自动生化分析仪检测K+批内CV分别为1.84%、0.89%,批间 CV 分别为2.08%、4.20%,Na+批内 CV 分别为0.05%、0.94%,Na+批间 CV 分别0.64%、0.81%,Cl-批内 CV 分别为0.88%、0.75%,Cl-批间 CV 分别为1.12%、1.21%,均满足CLIA88标准。正确度：检测结果在室间质评的允许范围。线性：K+ Y=1.0085X-0.0312,a=1.0085,r 2=0.99；Na+ Y=9998X-0.0055,a=0.9998,r 2=1；Cl- Y=0.9895X+1.8413,a=0.9895,r 2=0.99。可报告范围：K+0.68~11.8 mmol/L,Na+37~288.6 mmol/L,Cl-37.1~246.6 mmol/L。结论：奥林巴斯AU2700全自动生化分析仪检测电解质项目在精密度、准确度、线性范围等方面均达到了生物化学检验实验室的要求,可用于临床标本检测。
目的：評價奧林巴斯 AU2700全自動生化分析儀測定電解質項目的分析性能。方法：按照美國臨床實驗室標準化組織(CLSI)指南文件和其他相關文獻的實驗方案,分析奧林巴斯 AU2700全自動生化分析儀測定電解質項目的精密度、準確度、線性及可報告範圍等。結果：AU2700全自動生化分析儀檢測K+批內CV分彆為1.84%、0.89%,批間 CV 分彆為2.08%、4.20%,Na+批內 CV 分彆為0.05%、0.94%,Na+批間 CV 分彆0.64%、0.81%,Cl-批內 CV 分彆為0.88%、0.75%,Cl-批間 CV 分彆為1.12%、1.21%,均滿足CLIA88標準。正確度：檢測結果在室間質評的允許範圍。線性：K+ Y=1.0085X-0.0312,a=1.0085,r 2=0.99；Na+ Y=9998X-0.0055,a=0.9998,r 2=1；Cl- Y=0.9895X+1.8413,a=0.9895,r 2=0.99。可報告範圍：K+0.68~11.8 mmol/L,Na+37~288.6 mmol/L,Cl-37.1~246.6 mmol/L。結論：奧林巴斯AU2700全自動生化分析儀檢測電解質項目在精密度、準確度、線性範圍等方麵均達到瞭生物化學檢驗實驗室的要求,可用于臨床標本檢測。
목적：평개오림파사 AU2700전자동생화분석의측정전해질항목적분석성능。방법：안조미국림상실험실표준화조직(CLSI)지남문건화기타상관문헌적실험방안,분석오림파사 AU2700전자동생화분석의측정전해질항목적정밀도、준학도、선성급가보고범위등。결과：AU2700전자동생화분석의검측K+비내CV분별위1.84%、0.89%,비간 CV 분별위2.08%、4.20%,Na+비내 CV 분별위0.05%、0.94%,Na+비간 CV 분별0.64%、0.81%,Cl-비내 CV 분별위0.88%、0.75%,Cl-비간 CV 분별위1.12%、1.21%,균만족CLIA88표준。정학도：검측결과재실간질평적윤허범위。선성：K+ Y=1.0085X-0.0312,a=1.0085,r 2=0.99；Na+ Y=9998X-0.0055,a=0.9998,r 2=1；Cl- Y=0.9895X+1.8413,a=0.9895,r 2=0.99。가보고범위：K+0.68~11.8 mmol/L,Na+37~288.6 mmol/L,Cl-37.1~246.6 mmol/L。결론：오림파사AU2700전자동생화분석의검측전해질항목재정밀도、준학도、선성범위등방면균체도료생물화학검험실험실적요구,가용우림상표본검측。
Objective:Evaluation the analysis performance of Olympus AU2700 automatic biochemical Analyzer determined electrolytes proj ect.Methods:In accordance with United States clinical laboratory standards organization (CLSI) guidance document and other documents related to the experimental program,analyzes the precision,accuracy,linear range of Olympus AU2700 automatic biochemical analyzer determination electrolyte project.Results:AU2700 automatic biochemical analyzer examines in the K+ batch coefficient of variation respectively is 1.84% and 0.89%,the K+ inter-assay coefficients of variation respectively is 2.08% and 4.20%.The Na+ batch coefficient of variation respectively is 0.05% and 0.94%,the K+ inter-assay coefficients of variation respectively is 0.64% and 0.81%.The Cl- batch coefficient of variation respectively is 0.88% and 0.75%,the Cl- inter-assay coefficients of variation respectively is 1.12% and 1.21%.These results satisfies the CLIA88 standard.Accuracy:the detection result in the EQA the permissible range.Linearity:The linear regression equation of Potassium is Y=1.0085X-0.0312.a is 1.0085 and correlation coefficient is 0.99.Reportable range is 0.68~11.8 mmol/L.The linear regression equation of Sodium ions is Y=9998X-0.0055.a is 0.9998 and correlation co-efficient is 1.Reportable range is 37~288.6 mmol/L.The linear regression equation of Chloride ion is Y=0.9895X+1.8413.a is 0.9895 and correlation coefficient is 0.99.Reportable range is 37.1~246.6 mmol/L.Conclusion:Olympus AU2700 automatic biochemical analyzer examination electrolyte project has met the requirements of biochemical assay laboratory in precision,accuracy,linear range and other aspects,may be used in the clinical specimen examination.
