CAJ | 학술논문

目的：检测丰富环境对孕期应激大鼠子代海马糖皮质激素受体(Glucocorticoid Receptor, GR)的影响。方法：SD孕鼠随机分为2组,正常组和实验组。正常组动物不做任何处理,其子代断乳后在标准环境下喂养；实验组动物在孕第13~19天接受限制性应激,其子代断乳后一半在标准环境下喂养(构成应激组),另一半子代在丰富的环境下喂养(构成应激+丰富环境组)。所有子代出生后第35天被处死。用ABC免疫组织化学和 Western blot方法分别检测海马GR的表达。结果：GR 阳性产物广泛分布于海马各区神经元,应激组GR表达明显少于正常组；应激+丰富环境组海马 GR 的表达明显多于应激组,与正常组相比差别无统计学意义。结论：丰富环境能有效逆转孕期应激造成的子代海马GR的表达降低。
목적：검측봉부배경대잉기응격대서자대해마당피질격소수체(Glucocorticoid Receptor, GR)적영향。방법：SD잉서수궤분위2조,정상조화실험조。정상조동물불주임하처리,기자대단유후재표준배경하위양；실험조동물재잉제13~19천접수한제성응격,기자대단유후일반재표준배경하위양(구성응격조),령일반자대재봉부적배경하위양(구성응격+봉부배경조)。소유자대출생후제35천피처사。용ABC면역조직화학화 Western blot방법분별검측해마GR적표체。결과：GR 양성산물엄범분포우해마각구신경원,응격조GR표체명현소우정상조；응격+봉부배경조해마 GR 적표체명현다우응격조,여정상조상비차별무통계학의의。결론：봉부배경능유효역전잉기응격조성적자대해마GR적표체강저。
Obj ective:To observe the effects of enriched enviroment on the hippocampal glucocorticoid receptor of antenatal stressed offsprings.Methods:Pregnant SD rats were randomly separated into normal and experimental groups. The normal group accepted no stress.After weaning at postnatal day 21,their offsprings were given at the standard enviroment.The experimental group accepted the restraint stress at pregnancy day 13~19.The half of offsprings in experi-mental group were given the standard enviroment (stressed group).the other part was given the enriched enviroment treatment at postnatal day 22~35 (stress+enriched enviroment group).The GR expression in the hippocampus was observed by immunohistochemistry staining and western blot.Results:The expression of GR were found in the hippocampus of SD rats.Compared to the normal group,the GR expression of hippocampus in stressed group was meaningfully decreased. The GR expression of hippocampus in stress + enriched enviroment group was meaningfully higher than that of stressed group.Conclusion:Enriched enviroment can efficiently reverse the reduction of hippocampal GR expression in offsprings caused by antenatal stress.