CAJ | 학술논문

目的：探讨复合致病因素诱导的肝硬化大鼠TGF-α和TGF-β1改变及丹参素的保护作用。方法：取24只健康雄性SD大鼠,随机分为正常对照组(8只)、肝硬化组(8只)、丹参素干预组(8只)。采用复合致病因素法复制大鼠肝硬化模型,丹参素干预组采用丹参素进行干预[0.05 g/(kg·w),每日灌胃]。于实验第8周末取材；检测血浆谷丙转氨酶(ALT)、谷草转氨酶(AST)和内毒素水平；检测血浆和肝组织匀浆中肿瘤坏死因子-α(TNF-α)、转化生长因子-α(TGF-α)和转化生长因子-β1(TGF-β1)水平；肝组织切片行HE染色,观察病理学变化。结果：与正常对照组相比,肝硬化组脂肪变性明显,有典型的假小叶形成；血浆ALT、AST和内毒素水平显著升高(P<0.05)；血浆和肝组织匀浆TNF-α和TGF-β1水平也显著升高(P<0.05),而TGF-α水平无明显变化。与肝硬化组相比,丹参素干预组大鼠肝纤维化程度明显减轻；血浆ALT、AST和内毒素水平显著降低(P<0.05)；血浆和肝组织匀浆TNF-α和TGF-β1水平也显著降低(P<0.05),而TGF-α水平显著增高(P<0.05)。结论：在持续的肠源性内毒素作用下,TNF-α大量产生,TGF-α和TGF-β1之间失平衡,可能是复合致病因素诱导的大鼠肝硬化发病的重要机制,丹参素可以通过某种机制降低内毒素水平,减少TNF-α的产生,在一定程度上维持TGF-α和TGF-β1之间的平衡,对肝脏发挥促再生和保护作用。
목적：탐토복합치병인소유도적간경화대서TGF-α화TGF-β1개변급단삼소적보호작용。방법：취24지건강웅성SD대서,수궤분위정상대조조(8지)、간경화조(8지)、단삼소간예조(8지)。채용복합치병인소법복제대서간경화모형,단삼소간예조채용단삼소진행간예[0.05 g/(kg·w),매일관위]。우실험제8주말취재；검측혈장곡병전안매(ALT)、곡초전안매(AST)화내독소수평；검측혈장화간조직균장중종류배사인자-α(TNF-α)、전화생장인자-α(TGF-α)화전화생장인자-β1(TGF-β1)수평；간조직절편행HE염색,관찰병이학변화。결과：여정상대조조상비,간경화조지방변성명현,유전형적가소협형성；혈장ALT、AST화내독소수평현저승고(P<0.05)；혈장화간조직균장TNF-α화TGF-β1수평야현저승고(P<0.05),이TGF-α수평무명현변화。여간경화조상비,단삼소간예조대서간섬유화정도명현감경；혈장ALT、AST화내독소수평현저강저(P<0.05)；혈장화간조직균장TNF-α화TGF-β1수평야현저강저(P<0.05),이TGF-α수평현저증고(P<0.05)。결론：재지속적장원성내독소작용하,TNF-α대양산생,TGF-α화TGF-β1지간실평형,가능시복합치병인소유도적대서간경화발병적중요궤제,단삼소가이통과모충궤제강저내독소수평,감소TNF-α적산생,재일정정도상유지TGF-α화TGF-β1지간적평형,대간장발휘촉재생화보호작용。
Obj ective:To explore changes of TGF-αand TGF-β1 and protective effects of Danshensu in rats with liver cirrhosis induced by multiple pathogenic factors.Methods:The twenty-four male SD rats were randomly separated into normal control group(n=8),liver cirrhosis group(n=8)and Danshensu-treated group(n=8).Liver cirrhosis in rats was induced by multiple pathogenic factors.The rats in Danshensu-treated group were given by Danshensu[0.05 g/(kg·w),daily intragastric].The levels of ALT,AST and endotoxin in plasma were evaluated.The contents of TNF-α,TGF-αand TGF-β1 in plasma and in the liver tissues were detected.Histopathological changes of the liver were observed under microscope with the staining of HE.Results:Compared with normal control group,the levels of ALT,AST and en-dotoxin in plasma were significantly higher(P <0.05),and the levels of TNF-αand TGF-β1 in plasma and in the liver tissues were significantly higher(P<0.05),but the level of TGF-αwas no significant difference in liver cirrhosis group.In Danshensu-treated group,the pathological damage of hepatic tissue was significantly attenuated,and the levels of ALT,AST and endotoxin significantly decreased(P<0.05),and the levels of TNF-αand TGF-β1 in plasma and in the liver tissues were significantly decreased(P<0.05),however,the level of TGF-αwas significantly increased(P<0.05)compared with liver cirrhosis group.Conclusion:The findings suggest the imbalance of TGF-αand TGF-β1 may be the important pathogenesis of hepatic cirrhosis induced by multiple pathogenic factors because of the effects of sustained intestinal endotoxemia(IETM)and TNF-αin rats.Danshensu can reduce endotoxin and TNF-αvia certain mechanisms,and restore the balance between TGF-αand TGF-β1 in some degree,thereby Danshensu may exert a protective effect on hepatic cirrhosis induced by multiple pathogenic factors in rats.