CAJ | 학술논문

目的：采用HPLC法同步测定三七粉中人参皂苷Rg1、Rb1及三七皂苷R1含量。方法：采用SGE protecol C18（5μm，4.6×250mm）色谱柱，流动相为乙腈-水梯度洗脱（0~12min，19∶81；12~60min，19~36∶81~64），检测波长为203nm，流速1.0mL/min，进样量20μL。结果：人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1分别在25.625～430mg/L、26.875～410mg/L、10.625～170mg/L范围呈线性，相关系数r分别为0.9999、0.9998、0.9996；该方法重复性及回收率均符合要求。结论：本法用于同步测定三七粉中人参皂苷Rg1、Rb1及三七皂苷R1的含量，具有简便、准确、高效等特点。
목적：채용HPLC법동보측정삼칠분중인삼조감Rg1、Rb1급삼칠조감R1함량。방법：채용SGE protecol C18（5μm，4.6×250mm）색보주，류동상위을정-수제도세탈（0~12min，19∶81；12~60min，19~36∶81~64），검측파장위203nm，류속1.0mL/min，진양량20μL。결과：인삼조감Rg1、인삼조감Rb1、삼칠조감R1분별재25.625～430mg/L、26.875～410mg/L、10.625～170mg/L범위정선성，상관계수r분별위0.9999、0.9998、0.9996；해방법중복성급회수솔균부합요구。결론：본법용우동보측정삼칠분중인삼조감Rg1、Rb1급삼칠조감R1적함량，구유간편、준학、고효등특점。
Objective: This study has been to establish a method for synchronous determination of ginsenoside Rg1, ginsenoside Rb1 and notoginsenoside R1 in radix notoginseng powder. Method: SGE protecol C18(5μm,4.6×250mm)chromatographic column was used, with mixtures of acetonitrile and water as mobile phase in gradient mode (0~12min,19∶81; 12~60min, 19~36∶81~64), the wavelength was 203nm, the flow rate was 1.0mL/min, the injection volume was 20μL. Results: The linear range of the three saponins is 25.625 ~ 430mg/L, 26.875 ~ 410mg/L, 10.625 ~ 170mg/L respectively, and the correlation coefficient r is 0.9999, 0.9998, 0.9996 respectively. Repeatability and recovery rate are good. Conclusion: The presented method is a simple, accurate, efficient method for synchronous determination ginsenoside Rg1, ginsenoside Rb1 and notoginsenoside R1 in radix notoginseng powder.