CAJ | 학술논문

目的：比较研究纳米二氧化钛灌胃染毒对幼年和成年大鼠肝、肾组织抗氧化性能及组织中元素含量的影响。方法：将24只4周龄（幼年）和24只9周龄（成年）清洁级雄性SD大鼠按体重用随机数字表法分为8组（每组6只），每天1次灌胃，分别给予0、10、50、200 mg/kg纳米二氧化钛[（75±15） nm，锐钛矿]，染毒30 d后取肝、肾组织进行抗氧化性能指标和元素含量的检测。结果：200 mg/kg纳米二氧化钛染毒导致幼年大鼠的肝组织中总超氧化物歧化酶（ total superoxide dismutase ，T-SOD）活力和肾组织中还原型谷胱甘肽（ reduced glutathione ， GSH）/氧化型谷胱甘肽（ oxidized glutathione ， GSSG）比率明显升高，肝组织中Mo、Co、Mn、P元素含量和肾组织中Rb、Na元素含量明显降低。200 mg/kg纳米二氧化钛染毒导致成年大鼠的肝组织中GSH/GSSG比率和Rb元素含量明显升高，Na元素含量明显降低。成年大鼠肾组织中各指标无明显变化。结论：短期经口摄入纳米二氧化钛，可导致大鼠肝、肾组织的抗氧化能力升高和部分元素含量降低，肝组织反应更敏感，幼年大鼠比成年大鼠更易感。
목적：비교연구납미이양화태관위염독대유년화성년대서간、신조직항양화성능급조직중원소함량적영향。방법：장24지4주령（유년）화24지9주령（성년）청길급웅성SD대서안체중용수궤수자표법분위8조（매조6지），매천1차관위，분별급여0、10、50、200 mg/kg납미이양화태[（75±15） nm，예태광]，염독30 d후취간、신조직진행항양화성능지표화원소함량적검측。결과：200 mg/kg납미이양화태염독도치유년대서적간조직중총초양화물기화매（ total superoxide dismutase ，T-SOD）활력화신조직중환원형곡광감태（ reduced glutathione ， GSH）/양화형곡광감태（ oxidized glutathione ， GSSG）비솔명현승고，간조직중Mo、Co、Mn、P원소함량화신조직중Rb、Na원소함량명현강저。200 mg/kg납미이양화태염독도치성년대서적간조직중GSH/GSSG비솔화Rb원소함량명현승고，Na원소함량명현강저。성년대서신조직중각지표무명현변화。결론：단기경구섭입납미이양화태，가도치대서간、신조직적항양화능력승고화부분원소함량강저，간조직반응경민감，유년대서비성년대서경역감。
Objective:To compare the effect of TiO 2 nanoparticles on antioxidant function and element content of liver and kidney tissues in young and adult rats .Methods:Forty-eight SD male rats , half in 4-week (youth) old and half in 9-week (adult) old rats, were randomly divided into 8 groups, which were exposed to TiO2 nanoparticles [(75 ±15) nm, anatase] through intragastric administration at 0, 10, 50 and 200 mg/kg body weight every day for 30 days.The liver and kidney tissues were collected for antioxidant function and element content analysis .Results: 200 mg/kg TiO2 nanoparticles exposure significantly increased the liver total superoxide dismutase ( T-SOD ) activity and the kidney reduced glutathione (GSH)/oxidized glutathione (GSSG) ratios in young rats, and significantly decreased the liver Mo, Co, Mn and P contents and the kidney Rb and Na contents in young rats .200 mg/kg TiO2 nanoparticles exposure significantly increased GSH/GSSG ratios and Rb contents and decreased Na con-tents in the liver of adult rats .No significantly difference was found in antioxidant indexes and elements content in the kidney of adult rats between three experimental groups and control group .Conclusion:TiO2 nanoparticles can enhance the antioxidant capacity and decrease the elements content in rat liver and kidney tissues .The liver is the more sensitive target organ and the young animals are more susceptible to TiO2 nanoparticles toxicity by the oral routes .