北京大学学报（医学版）
北京大學學報（醫學版）
북경대학학보（의학판）
JOURNAL OF BEIJING MEDICAL UNIVERSITY(HEALTH SCIENCES)
2014年
3期
347-354
,共8页
槲皮素%体重%胰岛素样生长因子-1%肥胖症%大鼠
槲皮素%體重%胰島素樣生長因子-1%肥胖癥%大鼠
곡피소%체중%이도소양생장인자-1%비반증%대서
Quercetin%Body weight%Insulin-like growth factor-1%Obesity%Rats
目的：研究在孕期及哺乳期肥胖母鼠进行槲皮素干预后，对其F1代仔鼠体重和胰岛素样生长因子-1（insulin-like growth factors-1， IGF-1）mRNA表达的影响。方法：健康雌性SD大鼠断乳后，随机抽取8只喂养基础饲料作为空白对照组，其余喂养高脂饲料。当高脂饲料组大鼠平均体重超过空白对照组大鼠平均体重20％时，认为造模成功。将造模成功后的母鼠与健康雄鼠交配受孕，肥胖孕鼠随机分成4组，每组8只，整个孕期和哺乳期喂食高脂饲料，并分别以0 mg/kg体重（高脂对照组）、50 mg/kg体重、100 mg/kg体重、200 mg/kg体重剂量的槲皮素灌胃；空白对照组整个实验期喂食基础饲料并以溶剂灌胃。各组孕鼠自然分娩，记录F1代仔鼠出生体重及生后d7， d21，d56和d98体重，并检测F1代仔鼠肝组织IGF-1 mRNA的表达水平。结果：高脂对照组F1代仔鼠出生体重显著高于空白对照组。随槲皮素干预剂量增大，高脂对照组F1代仔鼠出生体重逐渐降低，接近空白对照组水平。槲皮素在一定程度上抑制F1代仔鼠肝组织IGF-1 mRNA的表达，持续到成年期，200 mg/kg体重效果最显著。结论：肥胖孕鼠F1代仔鼠出生体重显著增加，而孕期槲皮素干预可有效逆转F1代仔鼠体重，并控制F1代仔鼠断乳前体重过快增长。200 mg/kg体重的槲皮素能够有效抑制F1代仔鼠IGF-1 mRNA的表达，从而抑制细胞过度增殖和分化。
目的：研究在孕期及哺乳期肥胖母鼠進行槲皮素榦預後，對其F1代仔鼠體重和胰島素樣生長因子-1（insulin-like growth factors-1， IGF-1）mRNA錶達的影響。方法：健康雌性SD大鼠斷乳後，隨機抽取8隻餵養基礎飼料作為空白對照組，其餘餵養高脂飼料。噹高脂飼料組大鼠平均體重超過空白對照組大鼠平均體重20％時，認為造模成功。將造模成功後的母鼠與健康雄鼠交配受孕，肥胖孕鼠隨機分成4組，每組8隻，整箇孕期和哺乳期餵食高脂飼料，併分彆以0 mg/kg體重（高脂對照組）、50 mg/kg體重、100 mg/kg體重、200 mg/kg體重劑量的槲皮素灌胃；空白對照組整箇實驗期餵食基礎飼料併以溶劑灌胃。各組孕鼠自然分娩，記錄F1代仔鼠齣生體重及生後d7， d21，d56和d98體重，併檢測F1代仔鼠肝組織IGF-1 mRNA的錶達水平。結果：高脂對照組F1代仔鼠齣生體重顯著高于空白對照組。隨槲皮素榦預劑量增大，高脂對照組F1代仔鼠齣生體重逐漸降低，接近空白對照組水平。槲皮素在一定程度上抑製F1代仔鼠肝組織IGF-1 mRNA的錶達，持續到成年期，200 mg/kg體重效果最顯著。結論：肥胖孕鼠F1代仔鼠齣生體重顯著增加，而孕期槲皮素榦預可有效逆轉F1代仔鼠體重，併控製F1代仔鼠斷乳前體重過快增長。200 mg/kg體重的槲皮素能夠有效抑製F1代仔鼠IGF-1 mRNA的錶達，從而抑製細胞過度增殖和分化。
목적：연구재잉기급포유기비반모서진행곡피소간예후，대기F1대자서체중화이도소양생장인자-1（insulin-like growth factors-1， IGF-1）mRNA표체적영향。방법：건강자성SD대서단유후，수궤추취8지위양기출사료작위공백대조조，기여위양고지사료。당고지사료조대서평균체중초과공백대조조대서평균체중20％시，인위조모성공。장조모성공후적모서여건강웅서교배수잉，비반잉서수궤분성4조，매조8지，정개잉기화포유기위식고지사료，병분별이0 mg/kg체중（고지대조조）、50 mg/kg체중、100 mg/kg체중、200 mg/kg체중제량적곡피소관위；공백대조조정개실험기위식기출사료병이용제관위。각조잉서자연분면，기록F1대자서출생체중급생후d7， d21，d56화d98체중，병검측F1대자서간조직IGF-1 mRNA적표체수평。결과：고지대조조F1대자서출생체중현저고우공백대조조。수곡피소간예제량증대，고지대조조F1대자서출생체중축점강저，접근공백대조조수평。곡피소재일정정도상억제F1대자서간조직IGF-1 mRNA적표체，지속도성년기，200 mg/kg체중효과최현저。결론：비반잉서F1대자서출생체중현저증가，이잉기곡피소간예가유효역전F1대자서체중，병공제F1대자서단유전체중과쾌증장。200 mg/kg체중적곡피소능구유효억제F1대자서IGF-1 mRNA적표체，종이억제세포과도증식화분화。
Objective:To study the effect of quercetin given during pregnancy and lactation period of obese rats on weight and expression of insulin-like growth factors-1 ( IGF-1 ) mRNA of the offspring . Methods:In the study , 8 healthy weaning female SD rats were randomly selected to feed the basal diet and as blank control group , and the others were fed with high-fat diet.When the average weight of the high-fat diet rats was 20% as many as the average weight of the control group , we believed the model succeeded .Then the female SD rats were mated and the pregnant rats were randomly divided into 5 groups , 8 rats in each .They were fed with high fat diet during pregnancy and lactation , and the obese pregnant rats were respectively irrigated with 0 mg/kg body weight ( high-fat control ) , 50 mg/kg body weight,100 mg/kg body weight , and 200 mg/kg body weight quercetin .The blank control group was fed with basic diet throughout the experimental period .The birth weight , the weights during the development of d7, d21, d56, and d98, and the expressions of IGF-1 mRNA in livers tissue of the F1 generation were measured.Results:The average birth weight of the F1 generation of high fat diet (HFD) group was sig-nificantly higher than that of the blank control group .Decreased offspring birth weight was observed with the intervention of quercetin , which effect remained to the delectation .The quercetin also reduced the ex-pression of IGF-1 mRNA in livers of the F1 generation , and this effect remained to the adulthood .The 200 mg/kg body weight quercetin was most significantly effective .Conclusion:The averaged birth weight of the F1 generation of obese pregnant rats was significantly increased , and quercetin could effectively in-hibit the expression of IGF-1 mRNA in livers of F1 generation to decrease cell proliferation and cell differentiation.
