CAJ | 학술논문

目的：探索不同浓度原儿茶酸（ PCA ）对脂多糖（ LPS ）诱导的急性肺损伤（ ALI ）小鼠的保护作用，并探讨其对TLR4信号通路的作用。方法将60只小鼠分为6组：正常对照组、LPS模型组、PCA高剂量组、PCA中剂量组、PCA低剂量组、地塞米松阳性对照组。模型组以5mg· kg -1 LPS腹腔内注射诱导ALI。观察小鼠肺组织病理学变化；用BCA法检测肺泡灌洗液中总蛋白浓度；用ELISA检测肺泡灌洗液及血清中炎症因子TNF-α，IL-1β的表达，Western Blot 检测肺组织中TLR4蛋白的表达水平。结果与模型组相比，PCA治疗组肺组织损伤程度显著减轻，且与PCA给药浓度有关，高浓度组减轻显著；BALF及血清中TNF-α，IL-1β含量出现明显下降， PCA高剂量组差异显著（ P＜0．05）；BALF中蛋白浓度与模型组相比出现明显下降，PCA高剂量组差异显著（ P＜0．01）；TLR4蛋白表达量较LPS模型组相比显著降低（P＜0．01），并随浓度的增高而降低。结论 PCA对脂多糖所致急性肺损伤的保护作用与浓度有关，高浓度组作用显著，其作用机制可能与其抑制TLR4信号通路有关。
목적：탐색불동농도원인다산（ PCA ）대지다당（ LPS ）유도적급성폐손상（ ALI ）소서적보호작용，병탐토기대TLR4신호통로적작용。방법장60지소서분위6조：정상대조조、LPS모형조、PCA고제량조、PCA중제량조、PCA저제량조、지새미송양성대조조。모형조이5mg· kg -1 LPS복강내주사유도ALI。관찰소서폐조직병이학변화；용BCA법검측폐포관세액중총단백농도；용ELISA검측폐포관세액급혈청중염증인자TNF-α，IL-1β적표체，Western Blot 검측폐조직중TLR4단백적표체수평。결과여모형조상비，PCA치료조폐조직손상정도현저감경，차여PCA급약농도유관，고농도조감경현저；BALF급혈청중TNF-α，IL-1β함량출현명현하강， PCA고제량조차이현저（ P＜0．05）；BALF중단백농도여모형조상비출현명현하강，PCA고제량조차이현저（ P＜0．01）；TLR4단백표체량교LPS모형조상비현저강저（P＜0．01），병수농도적증고이강저。결론 PCA대지다당소치급성폐손상적보호작용여농도유관，고농도조작용현저，기작용궤제가능여기억제TLR4신호통로유관。
Objective To explore the protective effects of different concentrations of PCA on LPS-induced acute lung injury in mice,and to explore the role of TLR4 signaling pathway in ALI.Methods Sixty mice were divided into six groups:the normal group,the model group,high dose of PCA group,middle dose of PCA group,small dose of PCA group,dexamethasone group.The model group was in-duced by intraperitoneal injection of LPS with dose of 5mg/kg.Histopathological changes were observed in lung;Total protein concentration in bronchoalveolar lavage fluid was tested by BCA assay;Expression of Inflammatory cytokines TNF-αand IL-1βin bronchoalveolar lavage fluid and serum were detected by ELISA;Expression level of TLR 4 protein in lung tissue was detected by Western Blot .Results Compared with the model group ,different concentration of PCA treatment group was significantly reduced in lung injury degree ,especially in the high dose of PCA group;TNF-αand IL-1βlevels in BALF and serum marked decline in PCA groups ,and High dose groups were significantly;protein con-centration in BALF was marked decline compared with model group ,and high dose groups were significantly as well;expression of TLR4 pro-tein which was pretreated with PCA was significantly lower compared to LPS group ,and the content was decreased with increased PCA con-centration.Conclusion Protective effect of PCA on LPS-induced acute lung injury is concentration dependent and the high dose of PCA group has a significant effect to protect the lung from injury ,and the mechanism may be related to inhibition of TLR 4 signaling pathway .