医学临床研究
醫學臨床研究
의학림상연구
JOURNAL OF CLINICAL RESEARCH
2014年
4期
767-770
,共4页
彭颖%谢乐云%钟礼立%张兵%段招军%谢志萍
彭穎%謝樂雲%鐘禮立%張兵%段招軍%謝誌萍
팽영%사악운%종례립%장병%단초군%사지평
呼吸道感染/微生物学%湖南%病毒
呼吸道感染/微生物學%湖南%病毒
호흡도감염/미생물학%호남%병독
Respiratory Tract Infections/MI%HUNAN%Viruses
【目的】了解湖南地区儿童急性下呼吸道感染(ALRTI)住院儿童的病毒病原情况。【方法】收集2011年4月至2012年3月因ALRTI住院的儿童鼻咽抽吸物标本727份,采用RT-PCR以及 Nest-PCR方法检测常见的13种呼吸道病毒,包括人博卡病毒(HBoV ),腺病毒(ADV ),呼吸道合胞病毒(RSV ),人鼻病毒(HRV),副流感病毒1-4型(PIV1-4),流感病毒 A和B(IFVA/B),人偏肺病毒(HMPV),人冠状病毒NL63和HKU1(HCoV-NL63和 HCoV-HKU1)。并对所有阳性扩增产物进行基因测序证实。【结果】727份标本中有504份检出病毒,总检出率为69.3%(504/727),位居前三位的是:RSV(26.3%)、HRV(18.7%)、ADV (18.6%)。春夏季以及小于3岁以下患儿病毒检出率高,差异有统计学意义。病毒检出率的男女性别比无差异。两种或两种以上病毒混合感染率达38.9%。【结论】研究期间RSV以及 HRV是湖南地区ALRTI住院儿童的主要病原,但ADV检出率较往年明显增高,跃居第三位,具有重要提示意义,病毒之间混合感染率高。
【目的】瞭解湖南地區兒童急性下呼吸道感染(ALRTI)住院兒童的病毒病原情況。【方法】收集2011年4月至2012年3月因ALRTI住院的兒童鼻嚥抽吸物標本727份,採用RT-PCR以及 Nest-PCR方法檢測常見的13種呼吸道病毒,包括人博卡病毒(HBoV ),腺病毒(ADV ),呼吸道閤胞病毒(RSV ),人鼻病毒(HRV),副流感病毒1-4型(PIV1-4),流感病毒 A和B(IFVA/B),人偏肺病毒(HMPV),人冠狀病毒NL63和HKU1(HCoV-NL63和 HCoV-HKU1)。併對所有暘性擴增產物進行基因測序證實。【結果】727份標本中有504份檢齣病毒,總檢齣率為69.3%(504/727),位居前三位的是:RSV(26.3%)、HRV(18.7%)、ADV (18.6%)。春夏季以及小于3歲以下患兒病毒檢齣率高,差異有統計學意義。病毒檢齣率的男女性彆比無差異。兩種或兩種以上病毒混閤感染率達38.9%。【結論】研究期間RSV以及 HRV是湖南地區ALRTI住院兒童的主要病原,但ADV檢齣率較往年明顯增高,躍居第三位,具有重要提示意義,病毒之間混閤感染率高。
【목적】료해호남지구인동급성하호흡도감염(ALRTI)주원인동적병독병원정황。【방법】수집2011년4월지2012년3월인ALRTI주원적인동비인추흡물표본727빈,채용RT-PCR이급 Nest-PCR방법검측상견적13충호흡도병독,포괄인박잡병독(HBoV ),선병독(ADV ),호흡도합포병독(RSV ),인비병독(HRV),부류감병독1-4형(PIV1-4),류감병독 A화B(IFVA/B),인편폐병독(HMPV),인관상병독NL63화HKU1(HCoV-NL63화 HCoV-HKU1)。병대소유양성확증산물진행기인측서증실。【결과】727빈표본중유504빈검출병독,총검출솔위69.3%(504/727),위거전삼위적시:RSV(26.3%)、HRV(18.7%)、ADV (18.6%)。춘하계이급소우3세이하환인병독검출솔고,차이유통계학의의。병독검출솔적남녀성별비무차이。량충혹량충이상병독혼합감염솔체38.9%。【결론】연구기간RSV이급 HRV시호남지구ALRTI주원인동적주요병원,단ADV검출솔교왕년명현증고,약거제삼위,구유중요제시의의,병독지간혼합감염솔고。
[Objective] To understand the viral pathogen in hospitalized children with acute lower respira-tory tract infection(ALRTI) from 2011 to 2012 in Hunan province .[Methods]A total of 727 nasopharyngeal aspirate samples in hospitalized children due to ALRTI from April 2011 to March 2012 were collected .Reverse transcription polymerase chain reaction (RT-PCR) and Nest-PCR were used to detect 13 kinds of common re-spiratoryvirusesincludinghumanbocavirus(HBoV),adenovirus(ADV),respiratory syncytialvirus(RSV), human rhinovirus(HRV) ,parainfluenza 1-4(PIV1-4) ,influenza virus A(IFVA) ,influenza virus B(IFVB) , human metapneumovirus (HMPV) ,human coronaviruses NL63 (HCoV-NL63) and human coronaviruses HKU1(HCoV-HKU1) .The positive amplification products were confirmed by gene sequencing .[Results]Vi-ruses were detected in 504 of 727 specimens ,and the total detection rate was 69 .3% (504/727) .The first three viruses were RSV(26 .3% ) ,HRV(18 .7% ) and ADV(18 .6% ) .The high detection rate of viruses was found in spring and summer and children aged less than 3 years old ,and there was significant difference . There was no significant difference in the detection rate of viruses between males and females .The mixed in-fection rate of two or more than two kinds of viruses was 38 .9% .[Conclusion]During the study period ,respir-atory syncytial virus and rhinovirus are the main pathogens in hospitalized children with ALRTI of Hunan province .Compared with former years ,the detection rate of adenovirus is obviously increased ,and becomes the third common virus ,and has important significance .The mixed infection rate of viruses is high .