CAJ | 학술논문

目的探讨C反应蛋白(CRP)对多发性骨髓瘤细胞株U266细胞株生长的影响及其机制.方法将CRP(浓度为0、5、10、20 mg/L)与U266细胞株作用24 h后,血液分析仪检测细胞增生情况;采用反转录-聚合酶链反应(RT-PCR)方法检测凋亡相关基因Survivin和HSP-90α的表达.结果培养24 h后,U266细胞增牛率明显提高(P＜0.05),且与CRP浓度成正比,CRP浓度为20 mg/L时,细胞增生最明显;U266细胞表达Survivin、HSP-90α,其表达量随CRP浓度的增高而增高(P＜0.05).结论 CRP可促进U266细胞株增生,并通过调节凋亡抑制蛋白Survivin及HSP-90α表达的增高发挥其诱导U266细胞增生的作用.
목적 탐토C반응단백(CRP)대다발성골수류세포주U266세포주생장적영향급기궤제.방법 장CRP(농도위0、5、10、20 mg/L)여U266세포주작용24 h후,혈액분석의검측세포증생정황;채용반전록-취합매련반응(RT-PCR)방법 검측조망상관기인Survivin화HSP-90α적표체.결과배양24 h후,U266세포증우솔명현제고(P＜0.05),차여CRP농도성정비,CRP농도위20 mg/L시,세포증생최명현;U266세포표체Survivin、HSP-90α,기표체량수CRP농도적증고이증고(P＜0.05).결론 CRP가촉진U266세포주증생,병통과조절조망억제단백Survivin급HSP-90α표체적증고발휘기유도U266세포증생적작용.
Objective In the present study,we examined the expression changes of Survivin/HSP90α,in C-reactive protein(CRP)treated human multiple meyloma(MM) cell lies.Methotis The human MM cell lines were cultured for 3 days and incubated with human CRP(0.5,10,20 mg/L for 24 hours) and analyzed by blood analyser for proliferation ratio.The effects of CRP in MM cell lines in various concentrations on survivin/HSP-90α mRNA expression were examined by RT-PCR.Results Proliferation ratio of U266 cell line was significantly increased,and the expression of survivin/HSP-90α were upregulated(gene promoting cell survival)at mRNA levels in proportion to increased CRP concentrations.Conclusion These results demonstrated that proliferation ratio.survivin and HSP-90α could be regulated by CRP in MM cells.