CAJ | 학술논문

目的探讨甲异靛对白血病骨髓基质细胞干预白血病细胞增殖的影响.方法利用白血病骨髓单个核细胞培养骨髓基质细胞,并建立白血病细胞和骨髓基质的共培养体系.用锥虫蓝拒染实验测定甲异靛对共培养体系中白血病细胞增殖的影响；流式细胞术检测白血病细胞膜上CXCR4的表达.结果白血病骨髓基质细胞可抑制白血病细胞的增殖.低浓度的甲异靛(5 μmol/L)可促进白血病和骨髓基质细胞共培养体系中白血病细胞的增殖.白血病细胞膜异常高表达CXCR4,甲异靛可明显抑制HL-60细胞和原代白血病细胞膜上CXCR4的表达,骨髓基质细胞可以促进白血病细胞膜上CXCR4的表达.结论甲异靛可能通过下调白血病细胞膜上CXCR4的表达起抗白血病作用.
목적 탐토갑이전대백혈병골수기질세포간예백혈병세포증식적영향.방법 이용백혈병골수단개핵세포배양골수기질세포,병건립백혈병세포화골수기질적공배양체계.용추충람거염실험측정갑이전대공배양체계중백혈병세포증식적영향；류식세포술검측백혈병세포막상CXCR4적표체.결과 백혈병골수기질세포가억제백혈병세포적증식.저농도적갑이전(5 μmol/L)가촉진백혈병화골수기질세포공배양체계중백혈병세포적증식.백혈병세포막이상고표체CXCR4,갑이전가명현억제HL-60세포화원대백혈병세포막상CXCR4적표체,골수기질세포가이촉진백혈병세포막상CXCR4적표체.결론 갑이전가능통과하조백혈병세포막상CXCR4적표체기항백혈병작용.
Objective To observe the effect of meisoindigo on co-culture leukemic cells and bone marrow stromal cells.Methods Bone marrow stromal cells were cultured using bone marrow mononuclear cells from patients with leukemia,and built the co-culture leukemic cells and bone marrow stromal cells.Trypan-blue-exclusion test detected the proliferation of leukemic cells.The expression levels of membrane CXCR4 on leukemic cells were assessed by flow cytometric analysis.Results Bone marrow stromal cell of leukemia could inhibit leukemic cells proliferation,leukemic cells could be promoted their growth when exposed to low concentration (5 μmol/L) of meisoindigo.Abnormal high expression of CXCR4 in leukemic cells,and meisoindigo could markedly inhibit the expression of CXCR4 in HL-60 and del leukemic cells.Expression of CXCR4 in leukemic cells can up-regulated by bone marrow stromal cells from patients with leukemia.Conclusion Meisoindigo can down regulate expression of CXCR4 in leukeimic cells to antileukemic cells.