白血病·淋巴瘤
白血病·淋巴瘤
백혈병·림파류
JOURNAL OF LEUKEMIA & LYMPHOMA
2012年
12期
736-738
,共3页
白血病,淋巴细胞,急性%亚甲基四氢叶酸还原酶%多态性,单核苷酸
白血病,淋巴細胞,急性%亞甲基四氫葉痠還原酶%多態性,單覈苷痠
백혈병,림파세포,급성%아갑기사경협산환원매%다태성,단핵감산
Leukemia,lymphocytic,acute%Methylenetetrahydrofolate reductase%Polymorphisms,single nucleotide
目的 探讨亚甲基四氢叶酸还原酶(MTHFR)基因单核甘酸多态性与儿童急性淋巴细胞白血病(ALL)发病风险的关系.方法 分别收集45例ALL患儿(ALL组)及45名健康儿童(对照组)外周血各2 ml,提取基因组DNA,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法,检测MTHFR C677T和A1298C基因型,比较不同基因型对儿童ALL发病风险的影响.采用Logistic回归模型计算比值比(OR)和95％可信区间(95％CI).结果 对照组MTHFR 677CC、CT和TT基因型分布频率分别为31.1％(14/45)、51.1％(23/45)和17.7 ％(8/45),ALL组3种基因型分布频率分别为51.1％(23/45)、40.0％(18/45)和8.9％(4/45),两者比较差异有统计学意义(x2=7.48,P=0.04)；MTHFR 677 T等位基因在ALL组中的检出率为48.8 ％(22/45),在对照组中的检出率为69.9％(31/45);T等位基因携带者发生ALL的风险是CC基因型的0.4倍(95％CI0.21 ～0.83).对照组MTHFR 1298AA、AC和CC基因型分布频率分别为57.8％、40.0％和2.2％,ALL组3种基因型分布频率分别为18.8％、44.4％和6.8％,两者比较差异无统计学意义(x2=11.23,P=0.23)；MTHFR 1298 C等位基因在ALL组中的检出率为42.2％(19/45),在对照组中的检出率为51.1％(23/45)；C等位基因的存在并不会提高儿童发生ALL的风险(OR=1.3,95％CI0.21～0.83).结论 MTHFR 677 T等位基因的存在会显著降低儿童发生ALL的风险,而MTHFR 1298各基因型与儿童ALL的发生均无明显相关性.
目的 探討亞甲基四氫葉痠還原酶(MTHFR)基因單覈甘痠多態性與兒童急性淋巴細胞白血病(ALL)髮病風險的關繫.方法 分彆收集45例ALL患兒(ALL組)及45名健康兒童(對照組)外週血各2 ml,提取基因組DNA,應用聚閤酶鏈反應-限製性片段長度多態性(PCR-RFLP)法,檢測MTHFR C677T和A1298C基因型,比較不同基因型對兒童ALL髮病風險的影響.採用Logistic迴歸模型計算比值比(OR)和95％可信區間(95％CI).結果 對照組MTHFR 677CC、CT和TT基因型分佈頻率分彆為31.1％(14/45)、51.1％(23/45)和17.7 ％(8/45),ALL組3種基因型分佈頻率分彆為51.1％(23/45)、40.0％(18/45)和8.9％(4/45),兩者比較差異有統計學意義(x2=7.48,P=0.04)；MTHFR 677 T等位基因在ALL組中的檢齣率為48.8 ％(22/45),在對照組中的檢齣率為69.9％(31/45);T等位基因攜帶者髮生ALL的風險是CC基因型的0.4倍(95％CI0.21 ～0.83).對照組MTHFR 1298AA、AC和CC基因型分佈頻率分彆為57.8％、40.0％和2.2％,ALL組3種基因型分佈頻率分彆為18.8％、44.4％和6.8％,兩者比較差異無統計學意義(x2=11.23,P=0.23)；MTHFR 1298 C等位基因在ALL組中的檢齣率為42.2％(19/45),在對照組中的檢齣率為51.1％(23/45)；C等位基因的存在併不會提高兒童髮生ALL的風險(OR=1.3,95％CI0.21～0.83).結論 MTHFR 677 T等位基因的存在會顯著降低兒童髮生ALL的風險,而MTHFR 1298各基因型與兒童ALL的髮生均無明顯相關性.
목적 탐토아갑기사경협산환원매(MTHFR)기인단핵감산다태성여인동급성림파세포백혈병(ALL)발병풍험적관계.방법 분별수집45례ALL환인(ALL조)급45명건강인동(대조조)외주혈각2 ml,제취기인조DNA,응용취합매련반응-한제성편단장도다태성(PCR-RFLP)법,검측MTHFR C677T화A1298C기인형,비교불동기인형대인동ALL발병풍험적영향.채용Logistic회귀모형계산비치비(OR)화95％가신구간(95％CI).결과 대조조MTHFR 677CC、CT화TT기인형분포빈솔분별위31.1％(14/45)、51.1％(23/45)화17.7 ％(8/45),ALL조3충기인형분포빈솔분별위51.1％(23/45)、40.0％(18/45)화8.9％(4/45),량자비교차이유통계학의의(x2=7.48,P=0.04)；MTHFR 677 T등위기인재ALL조중적검출솔위48.8 ％(22/45),재대조조중적검출솔위69.9％(31/45);T등위기인휴대자발생ALL적풍험시CC기인형적0.4배(95％CI0.21 ～0.83).대조조MTHFR 1298AA、AC화CC기인형분포빈솔분별위57.8％、40.0％화2.2％,ALL조3충기인형분포빈솔분별위18.8％、44.4％화6.8％,량자비교차이무통계학의의(x2=11.23,P=0.23)；MTHFR 1298 C등위기인재ALL조중적검출솔위42.2％(19/45),재대조조중적검출솔위51.1％(23/45)；C등위기인적존재병불회제고인동발생ALL적풍험(OR=1.3,95％CI0.21～0.83).결론 MTHFR 677 T등위기인적존재회현저강저인동발생ALL적풍험,이MTHFR 1298각기인형여인동ALL적발생균무명현상관성.
Objective To investigate the relationship between genetic polymorphism of methylenetetrahydrofolate reductase (MTHFR) and the risk of childhood acute lymphocytic leukemia (ALL).Methods 45 patients with ALL and a cohort of 45 matched healthy children were included,and DNA was extracted from their peripheral blood.PCR-RFLP was used to determine the genotypes of MTHFR C677T and A1298C.The adjusted odds tatio (OR) and 95 ％ confidence interwal (CI) were calculated using unconditional logistic regression model.Results The frequency of MTHFR 677 CC,CT and TT genetypes were 31.1％ (14/45),51.1％ (23/45) and 17.7 ％ (8/45) in controls and 51.1％ (23/45),40.0 ％ (18/45) and 8.9 ％ (4/45)in ALL,respectively (x2 =7.48,P =0.04).The frequency of MTHFR 677 T allele were 69.9 ％ (31/45) in controls and 48.8 ％ (22/45) in ALL.The MTHFR 677 T allele had an decreased risk in ALL compared with CC genetype (OR =0.4,95 ％ CI 0.21-0.83).The frequency of MTHFR 1298 AA,AC and CC genetypes were 57.8 ％,40.0 ％ and 2.2 ％ in controls and 18.8 ％,44.4 ％ and 6.8 ％ in ALL,respectively (x2 =11.23,P=0.23).The frequency of MTHFR 1298 C allele were 51.1％ (23/45) in controls and 42.2 ％ (19/45) in ALL.No significant association between the MTHFR 1298 polymorphism and the risk of ALL (OR =1.3,95 ％ CI 0.21-0.83).Conclusion MTHFR 677 polymorphism could significantly decrease the risk of developing childhood ALL,whereas MTHFR 1298 don' t significantly affect the risk of ALL.
