CAJ | 학술논문

目的探究8-氯腺苷酸(8-Cl-cAMP)对多发性骨髓瘤(MM)细胞的作用和三氧化二砷(As2O3)对其影响.方法以MM细胞株RPMI 8226和U266细胞为体外模型,应用形态学和流式细胞术检测细胞DNA含量分布和细胞凋亡.以罗丹明染色检测线粒体跨膜电位,并以金氏公式评价8-Cl-cAMP和As2O3之间的协同效应.结果 1～30 mol/L 8-Cl-cAMP能够明显抑制RPMI 8226和U266细胞的生长,并显著降低其细胞活力,呈时间和浓度依赖性.细胞形态学和流式细胞术分析显示,8-Cl-cAMP诱导骨髓瘤细胞凋亡并伴有线粒体跨膜电位的崩塌.As2O3能促进RPMI 8226细胞凋亡,但与8-Cl-cAMP无协同作用.结论8-Cl-cAMP能够在体外有效地抑制MM细胞生长并诱导其凋亡,线粒体可能是8-Cl-cAMP诱导凋亡的靶点之一,As2O3催化了这个凋亡过程.
목적 탐구8-록선감산(8-Cl-cAMP)대다발성골수류(MM)세포적작용화삼양화이신(As2O3)대기영향.방법 이MM세포주RPMI 8226화U266세포위체외모형,응용형태학화류식세포술검측세포DNA함량분포화세포조망.이라단명염색검측선립체과막전위,병이금씨공식평개8-Cl-cAMP화As2O3지간적협동효응.결과 1～30 mol/L 8-Cl-cAMP능구명현억제RPMI 8226화U266세포적생장,병현저강저기세포활력,정시간화농도의뢰성.세포형태학화류식세포술분석현시,8-Cl-cAMP유도골수류세포조망병반유선립체과막전위적붕탑.As2O3능촉진RPMI 8226세포조망,단여8-Cl-cAMP무협동작용.결론8-Cl-cAMP능구재체외유효지억제MM세포생장병유도기조망,선립체가능시8-Cl-cAMP유도조망적파점지일,As2O3최화료저개조망과정.
Objective To investigate the response of multiple myeloma (MM) cells to 8-chloroadenosine 3',5'-monophosphate (8-Cl-cAMP) and the impact of arsenic trioxide (As2O3) on the above reaction.Methods MM-derived cell lines RPMI8226 and U266 were used as in vitro models.Cell apoptosis was evaluated according to cellular morphology and DNA content measured by flow cytometry.Meanwhile,rhodamine 123 (Rh123) staining and flow cytometry assay were used to detect the changes of mitochondrial transmembrane potentials (△ψm) in MM cells before and after the treatment.The synergic effects of 8-Cl-cAMP and As2O3 were evaluated by King' s formula.Results The 8-Cl-cAMP could induce growth inhibition of RPMI8226 and U266 cells in dose and time-related manners.The 8-Cl-cAMP could trigger apoptosis and △ψm collapse in MM cells through cellular morphology and flow cytometry analysis.As2O3 accelerated 8-Cl-cAMP-mediated apoptosis of RPMI8226 cells,but there were few synergic effects observed.Conclusion 8-Cl-cAMP could induce cell proliferation inhibition and apoptosis in MM cells.Mitochondria may be one of targets in 8-Cl-cAMP-mediated apoptosis.Furthermore,As2O3 catalyzes 8-Cl-cAMP-induced apoptosis.