CAJ | 학술논문

目的观察大鼠严重烧伤后胃肠运动功能改变及肠道平滑肌细胞表型转化.方法正常对照组大鼠麻醉后脱毛不烫伤,烧伤组制备30% TBSA Ⅲ度烧伤大鼠模型,依照伤后时相点的不同分为伤后2 h、6 h、12 h、24 h、48、72 h组,按时相点检测炭末推进率,观察严重烧伤后胃肠运动功能的改变;按时相点取材结肠标本,免疫组织化学染色,图像分析,观察严重烧伤后肠道平滑肌细胞表型转化的改变.结果伤后不同时相点,炭末推进率较正常对照组(74.20±4.28)%均显著降低,伤后6 h最低为(38.52±3.93)%,黏膜下肌层平滑肌细胞的α-平滑肌肌动蛋白在伤后6 h表达的阳性单位值较正常对照组(25.11±5.65)明显下降,伤后24 h表达的阳性单位值最低(10.56±2.68),并持续到伤后72 h且黏膜下肌层平滑肌组织明显增生,而肠壁组织平滑肌的α-平滑肌肌动蛋白表达无明显变化.结论大鼠严重烧伤后胃肠运动功能明显抑制;严重烧伤可引起肠道黏膜下层平滑肌细胞表型的改变,伤后胃肠运动功能抑制可能是由于肠道黏膜下层平滑肌细胞发生表型转化,α-平滑肌肌动蛋白可能在大鼠严重烧伤后起下调炎症反应及保护细胞的作用.
목적 관찰대서엄중소상후위장운동공능개변급장도평활기세포표형전화.방법 정상대조조대서마취후탈모불탕상,소상조제비30% TBSA Ⅲ도소상대서모형,의조상후시상점적불동분위상후2 h、6 h、12 h、24 h、48、72 h조,안시상점검측탄말추진솔,관찰엄중소상후위장운동공능적개변;안시상점취재결장표본,면역조직화학염색,도상분석,관찰엄중소상후장도평활기세포표형전화적개변.결과 상후불동시상점,탄말추진솔교정상대조조(74.20±4.28)%균현저강저,상후6 h최저위(38.52±3.93)%,점막하기층평활기세포적α-평활기기동단백재상후6 h표체적양성단위치교정상대조조(25.11±5.65)명현하강,상후24 h표체적양성단위치최저(10.56±2.68),병지속도상후72 h차점막하기층평활기조직명현증생,이장벽조직평활기적α-평활기기동단백표체무명현변화.결론 대서엄중소상후위장운동공능명현억제;엄중소상가인기장도점막하층평활기세포표형적개변,상후위장운동공능억제가능시유우장도점막하층평활기세포발생표형전화,α-평활기기동단백가능재대서엄중소상후기하조염증반응급보호세포적작용.
Objective To observe expression of α-MC-actin in the intestinal smooth muscle cell and to find out the role of phenotype switching after severe burn injury.Methods Fifty-six rats were divided into control group and burn groups respectedly. A rat model of 30% full-thickness burn was established in the burn group and the burn group are divided into six sub-groups according to different time from 2h to 72h post-burn. Carbo medicinalis propulsive was used for detecting intestinal propulsive rate at various phase post-burn as to know the change of intestinal movement. Immunohistochemical staining method and color image analysis were used to observe the expression of α-MC-actin of the post-burn smooth muscle. Results Carbo medicinalis propulsive rate was detected at various phase post-burn, much lower than that of the control group(74.20±4.28)%, and the lowest in 6 h(38.52±3.93)%.The expression of α-MC-Actin of the smooth muscle under intestinal mucosa were detected at various phase post-burn much lowered than that of the control group(25.11±5.65),decreased at 6h post-burn ,and the lowest in 24 h(10.56±2.68) , and lasted for 72 h post-burn and accompanied the marked expansion of the smooth muscle under intestinal mucosa. But the expression of α-MC-actin of the intestinal wall showed no change. Conclusions Severe scald in rats can cause phenotype switching of the SMCS under intestinal mucosa. The function of gastrointestinal motility could obviously be inhibited by severe burns. The SMC under intestinal mucosa have phenotype switch and might be the key factor. The expression may relieve inflammatory reaction and protect cell.