口腔生物医学
口腔生物醫學
구강생물의학
ORAL BIOMEDICINE
2014年
1期
15-18
,共4页
王利娟%俞艳%曹灵%薛晶%张光东%于金华
王利娟%俞豔%曹靈%薛晶%張光東%于金華
왕리연%유염%조령%설정%장광동%우금화
牙髓干细胞%青霉素%链霉素%炎症%增殖%分化
牙髓榦細胞%青黴素%鏈黴素%炎癥%增殖%分化
아수간세포%청매소%련매소%염증%증식%분화
Dental pulp stem cells%Penicillin-Streptomycin%Inflammation%Proliferation%Differentiation
目的:探讨青霉素和链霉素对炎症牙髓干细胞的增殖、成牙成骨分化能力及肿瘤坏死因子α( tumor necrosis factor α, TNF-α)表达的影响。方法:采用酶消化法分离培养炎症牙髓干细胞,采用甲基噻唑基四唑(MTT)比色法、碱性磷酸酶活性检测、Western blot及实时定量RT-PCR等方法分析青霉素和链霉素作用于炎症牙髓干细胞后,其增殖和成牙成骨分化指标(核心结合因子、牙本质涎蛋白/牙本质涎磷蛋白、骨钙素)及TNF-α表达的变化。结果:青霉素和链霉素作用于炎症牙髓干细胞后,与未加抗生素的培养组细胞的增殖能力及成骨/成牙相关蛋白、基因的表达无明显差异(P>0.05),而TNF-α的表达则低于普通培养组(P<0.01)。结论:青霉素和链霉素降低炎症牙髓干细胞的炎性因子TNF-α表达,对细胞的增殖及成牙/成骨分化能力无明显影响。
目的:探討青黴素和鏈黴素對炎癥牙髓榦細胞的增殖、成牙成骨分化能力及腫瘤壞死因子α( tumor necrosis factor α, TNF-α)錶達的影響。方法:採用酶消化法分離培養炎癥牙髓榦細胞,採用甲基噻唑基四唑(MTT)比色法、堿性燐痠酶活性檢測、Western blot及實時定量RT-PCR等方法分析青黴素和鏈黴素作用于炎癥牙髓榦細胞後,其增殖和成牙成骨分化指標(覈心結閤因子、牙本質涎蛋白/牙本質涎燐蛋白、骨鈣素)及TNF-α錶達的變化。結果:青黴素和鏈黴素作用于炎癥牙髓榦細胞後,與未加抗生素的培養組細胞的增殖能力及成骨/成牙相關蛋白、基因的錶達無明顯差異(P>0.05),而TNF-α的錶達則低于普通培養組(P<0.01)。結論:青黴素和鏈黴素降低炎癥牙髓榦細胞的炎性因子TNF-α錶達,對細胞的增殖及成牙/成骨分化能力無明顯影響。
목적:탐토청매소화련매소대염증아수간세포적증식、성아성골분화능력급종류배사인자α( tumor necrosis factor α, TNF-α)표체적영향。방법:채용매소화법분리배양염증아수간세포,채용갑기새서기사서(MTT)비색법、감성린산매활성검측、Western blot급실시정량RT-PCR등방법분석청매소화련매소작용우염증아수간세포후,기증식화성아성골분화지표(핵심결합인자、아본질연단백/아본질연린단백、골개소)급TNF-α표체적변화。결과:청매소화련매소작용우염증아수간세포후,여미가항생소적배양조세포적증식능력급성골/성아상관단백、기인적표체무명현차이(P>0.05),이TNF-α적표체칙저우보통배양조(P<0.01)。결론:청매소화련매소강저염증아수간세포적염성인자TNF-α표체,대세포적증식급성아/성골분화능력무명현영향。
Objective:To observe the effects of Penicillin-Streptomycin on the proliferation , odonto-and osteogenic differentiation and the expression of the inflammatory cytokines of inflamed human dental pulp stem cells (iDPSCs) in vitro.Methods:iDPSCs were isolated from inflamed human dental pulp and cultured respectively in alpha minimum essential medium (α-MEM) or containing 100 U/mL Penicillin-Streptomycin.The proliferation ability of iDPSCs was evaluated by MTT colorimetric assay .Alkaline phosphotase (ALP) activity, real-time RT-PCR and Western blot were used to examine the odonto /osteogenic potential-the the expression of RUNX2/RUNX2,DSP/DSPP,OCN/OCN and the expression of TNF-αof iDPSCs.Results:MTT assay showed that 100U/ml Penicillin-Strepto-mycin had no effect on the proliferation of iDPSCs , ALP activity assay , real-time RT-PCR and Western blot showed that there was no difference between Penicillin-Streptomycin group and α-MEM group ( P>0.05).The expression of TNF-αdecreased in Penicillin-Streptomycin group (P<0.01).Conclusions:Penicillin-Streptomycin significantly suppressed the expression of inflammatory cytokines in inflammatory dental pulp stem cells , and has no noticeable effect on the proliferation and differentiation of the cells .