CAJ | 학술논문

利用光学显微镜、分子生物学方法,对分离自广西北部湾海域的三角棘原甲藻 Prorocentrum triestinum BBW-02藻株的形态特征进行了描述,并探讨了其分子系统进化关系。三角棘原甲藻 BBW-02细胞呈长卵形或披针形,细胞长20~26μm,宽10~14μm,顶刺形如矛尖,刺丝胞孔数量较少,排列不规则,主要分布于壳板边缘,距离细胞底端1/3处壳板两侧各有2个近于对称排列的刺丝胞孔。18S rDNA序列同源检索和系统进化分析表明,三角棘原甲藻BBW-02与其他地理株系的三角棘原甲藻聚为一支,亲缘关系密切,遗传差异仅为0.000~0.001。与其他种类相比,三角棘原甲藻BBW-02与同属中的P. gracile亲缘关系最近。
이용광학현미경、분자생물학방법,대분리자엄서북부만해역적삼각극원갑조 Prorocentrum triestinum BBW-02조주적형태특정진행료묘술,병탐토료기분자계통진화관계。삼각극원갑조 BBW-02세포정장란형혹피침형,세포장20~26μm,관10~14μm,정자형여모첨,자사포공수량교소,배렬불규칙,주요분포우각판변연,거리세포저단1/3처각판량측각유2개근우대칭배렬적자사포공。18S rDNA서렬동원검색화계통진화분석표명,삼각극원갑조BBW-02여기타지리주계적삼각극원갑조취위일지,친연관계밀절,유전차이부위0.000~0.001。여기타충류상비,삼각극원갑조BBW-02여동속중적P. gracile친연관계최근。
Prorocentrum triestinum BBW-02 strain, isolated from the Beibu Gulf of Guangxi province, was described on the basis of morphological characteristics and phylogenetic evolution relationship using light microscopy and molecular sequencing. The cells are lanceolate or heart-shaped, 20-26 μm long and 10-14 μm wide. The anterior spine is spearhead-shaped. The thichocysts of the surface are few, and mainly distribute around the margin irregularly. Two thichocysts distribute approximate symmetrically in the two-third of the theca. Homologous retrieval and system evolution analysis showed that P. triestinum BBW-02 was closely related to the other analysed P. triestinum. Compared with other species, P. triestinum BBW-02 had the most intimate relationship with P. gracile.