CAJ | 학술논문

目的：在已有的实验基础上，进一步寻求α7尼古丁受体（α7nAChR）介导100 Hz电针（EA）调节自然杀伤细胞（NK）细胞活性的新证据。方法应用α7nAChR抗体和特异性的α7nAChR拮抗剂α-银环蛇毒（α-Bgt），探讨α7nAChR在EA对NK细胞活性中的作用。结果与100 Hz EA组相比，100 Hz EA＋α7nAChR抗体组脾脏NK细胞活性明显增强，差异有统计学意义（P＜0．05）；与100 Hz EA 组相比，1．5和3．0μg α-Bgt ＋100Hz EA组均 NK 细胞活性明显增强，差异有统计学意义（P＜0．01）。结论α7nAChR介导了100 Hz EA调节免疫功能的作用，这为进一步明确和扩展EA的临床应用提供理论和实践依据。
목적：재이유적실험기출상，진일보심구α7니고정수체（α7nAChR）개도100 Hz전침（EA）조절자연살상세포（NK）세포활성적신증거。방법응용α7nAChR항체화특이성적α7nAChR길항제α-은배사독（α-Bgt），탐토α7nAChR재EA대NK세포활성중적작용。결과여100 Hz EA조상비，100 Hz EA＋α7nAChR항체조비장NK세포활성명현증강，차이유통계학의의（P＜0．05）；여100 Hz EA 조상비，1．5화3．0μg α-Bgt ＋100Hz EA조균 NK 세포활성명현증강，차이유통계학의의（P＜0．01）。결론α7nAChR개도료100 Hz EA조절면역공능적작용，저위진일보명학화확전EA적림상응용제공이론화실천의거。
Objective On the basis of previous experiments ,we try further to seek the new evidences of α7nAChR participating in 100 Hz electroacupuncture (EA ) regulating natural killer (NK ) cells activity .Methods α7nAChR antibody and the specificα7nAChR antagonist α-bungarotoxin (α-Bgt) were used to study the effect of α7nAChR on NK cell activity in 100 Hz EA mice .Re-sults Compared with 100 Hz EA group ,100 Hz EA plus α7nAChR-Ab group showed a significant enhanced in NK cell activity (P<0 .05) .100 Hz EA treated with 1 .5 or 3 .0 μg α-Bgt groups had higher NK cell activity than 100 Hz EA group(P<0 .01) . Conclusion α7nAChR is involved in 100 Hz EA modulation for immune function ,which provide the theoretical and practical basis for further clarifying and expanding the clinical application of EA .