CAJ | 학술논문

目的：探讨氟灭酸（FFA ）对微动脉平滑肌细胞间缝隙连接的影响。方法采用全细胞膜片钳技术观察FFA对微动脉段上平滑肌细胞膜电容（Cinput ）、膜电导（Ginput ）或膜电阻（Rinput ）的影响。结果 FFA可以浓度依赖和可逆的降低微动脉段上平滑肌细胞的Cinput和Ginput （或者增加 Rinput ）。FFA抑制肠系膜动脉（M A ）和脑动脉（BA ）平滑肌细胞Ginput的IC50分别为56、33μmol/L ，微动脉间比较差异无统计学意义（P＞0．05）。FFA浓度大于或等于300μmol/L时，MA和BA微动脉段上平滑肌细胞的Cinput 、Ginput或Rinput与相应单个平滑肌细胞十分接近。结论 FFA可以浓度依赖和可逆的阻断微动脉平滑肌细胞间缝隙连接， FFA的浓度大于或等于300μmol/L时可以完全阻断微动脉平滑肌细胞间的缝隙连接。FFA对M A和BA微动脉细胞间缝隙连接具有同源性。
목적：탐토불멸산（FFA ）대미동맥평활기세포간봉극련접적영향。방법채용전세포막편겸기술관찰FFA대미동맥단상평활기세포막전용（Cinput ）、막전도（Ginput ）혹막전조（Rinput ）적영향。결과 FFA가이농도의뢰화가역적강저미동맥단상평활기세포적Cinput화Ginput （혹자증가 Rinput ）。FFA억제장계막동맥（M A ）화뇌동맥（BA ）평활기세포Ginput적IC50분별위56、33μmol/L ，미동맥간비교차이무통계학의의（P＞0．05）。FFA농도대우혹등우300μmol/L시，MA화BA미동맥단상평활기세포적Cinput 、Ginput혹Rinput여상응단개평활기세포십분접근。결론 FFA가이농도의뢰화가역적조단미동맥평활기세포간봉극련접， FFA적농도대우혹등우300μmol/L시가이완전조단미동맥평활기세포간적봉극련접。FFA대M A화BA미동맥세포간봉극련접구유동원성。
Objective To investigate the influence of flufenamic acid (FFA) on gap junction intercellular communication in vascu-lar smooth muscle cells(VSMC) in situ of acutely isolated arteriole segments .Methods Whole-cell patch clamp recordings were used to study the effects of FFA on membrane input capacitance (Cinput ) ,membrane input conductance(Ginput ) or membrane input re-sistance(Rinput ) of VSMCs embedded in arteriole segments .Results FFA concentration-dependently and reversibly suppressed Ginput or increased Rinput ,with an IC50 of 56 and 33μmol/L in acutely isolated mesenteric artery(MA) and brain artery(BA) segments re-spectively .There was not significant difference between MA and BA (P> 0 .05) .After application of FFA (≥ 300 μmol/L) ,the Cinput ,Ginput and Rinput of the in situ cells were very close to the respective dispersed cell in MA and BA .Conclusion FFA is a reversi-ble gap junction blocker ,achieving a complete electrical isolation of the recorded VSMC at ≥300 μmol/L .FFA suggesting a homo-geneous property of the gap junctions between MA and BA .