CAJ | 학술논문

目的：探讨星形肢质细胞上调基因1（AEG-1）在非小细胞肺癌（NSCLC）中的表达及其临床病理意义。方法采用RT-PCR和Western blot方法检测NSCLC和癌旁组织中AEG-1的表达，进一步用免疫组织化学的方法检测87例NSCLC组织和54例非癌肺组织中AEG-1的表达。结果 RT-PCR和Western blot表明AEG-1在NSCLC中表达水平高于相应癌旁组织，免疫组织化学结果表明NSCLC组织中AEG-1的高表达率为52．9％，显著高于非癌肺组织中的29．6％，两者之间差异有统计学意义（P＝0．007）；AEG-1表达强度与肿瘤T、N分期呈正相关（P＜0．05）。结论 AEG-1在 NSCLC中表达增高，有望作为诊断NSCLC的生物标记物。
목적：탐토성형지질세포상조기인1（AEG-1）재비소세포폐암（NSCLC）중적표체급기림상병리의의。방법채용RT-PCR화Western blot방법검측NSCLC화암방조직중AEG-1적표체，진일보용면역조직화학적방법검측87례NSCLC조직화54례비암폐조직중AEG-1적표체。결과 RT-PCR화Western blot표명AEG-1재NSCLC중표체수평고우상응암방조직，면역조직화학결과표명NSCLC조직중AEG-1적고표체솔위52．9％，현저고우비암폐조직중적29．6％，량자지간차이유통계학의의（P＝0．007）；AEG-1표체강도여종류T、N분기정정상관（P＜0．05）。결론 AEG-1재 NSCLC중표체증고，유망작위진단NSCLC적생물표기물。
Objective To investigate the expression of astrocyte elevated gene 1(AEG-1) in non-small cell lung cancer(NSCLC) and its clinicalpathological significance .Methods The expression of AEG-1 in NSCLC tissues and adjacent normal lung tissues was detected by RT-PCR and Western blot .The expression of AEG-1 in 87 NSCLC samples and 54 non-cancerous lung tissues was ex-amined with immunohistochemistry .Results RT-PCR and Western blot indicated that AEG-1 was more expressed in NSCLC tis-sues compared with adjacent normal lung tissues .The positive rate of AEG-1 in NSCLC tissues was 52 .9% ,which was significantly higher than that in non-cancerous lung tissues .The difference between the two groups was significant (P=0 .007) .Relevance was analyzed between the expression of AEG-1 and clinicalpathological characteristic .The expression of AEG-1 was positively correlated with T stage and N stage(P<0 .05) .Conclusion AEG-1 was specifically up-regulated in NSCLC tissues compared with non-can-cerous lung tissues ,suggesting that AEG-1 may play an important role in tumor development and progression ,and could be identi-fied as a biomarker for diagnosis of NSCLC .