食品工程
食品工程
식품공정
FOOD ENGINEERING
2012年
1期
49-51
,共3页
金露%张妤%虞选柯%郭应建%陆雪娇%关允诗%黄光荣
金露%張妤%虞選柯%郭應建%陸雪嬌%關允詩%黃光榮
금로%장여%우선가%곽응건%륙설교%관윤시%황광영
高效液相色谱%月饼%防腐剂
高效液相色譜%月餅%防腐劑
고효액상색보%월병%방부제
high performance liquid chromatography%mooncake%antimicrobial reagent
利用高效液相色谱法测定5种月饼样品中的3种常见防腐剂苯甲酸、山梨酸和脱氢乙酸的含量。样品经提取、脱蛋白、膜过滤后,用高效液相色谱进行测定。结果表明,3种防腐剂的测定线性相关性均在0.99以上,检测线性范围为10μg/mL～200μg/mL。该方法应用于5种月饼中苯甲酸、山梨酸和脱氢乙酸的测定结果表明,5种样品均检出山梨酸,3种样品检出有脱氢乙酸,均未检出苯甲酸。
利用高效液相色譜法測定5種月餅樣品中的3種常見防腐劑苯甲痠、山梨痠和脫氫乙痠的含量。樣品經提取、脫蛋白、膜過濾後,用高效液相色譜進行測定。結果錶明,3種防腐劑的測定線性相關性均在0.99以上,檢測線性範圍為10μg/mL～200μg/mL。該方法應用于5種月餅中苯甲痠、山梨痠和脫氫乙痠的測定結果錶明,5種樣品均檢齣山梨痠,3種樣品檢齣有脫氫乙痠,均未檢齣苯甲痠。
이용고효액상색보법측정5충월병양품중적3충상견방부제분갑산、산리산화탈경을산적함량。양품경제취、탈단백、막과려후,용고효액상색보진행측정。결과표명,3충방부제적측정선성상관성균재0.99이상,검측선성범위위10μg/mL～200μg/mL。해방법응용우5충월병중분갑산、산리산화탈경을산적측정결과표명,5충양품균검출산리산,3충양품검출유탈경을산,균미검출분갑산。
Three common kinds of antimicrobial reagents in mooncakes,benzoic acid,sorbic acid and dehydroacetic acid,were determined with high performance liquid chromatography（HPLC）.After extracting,removal of proteins and membrane filtration,samples were tested with HPLC.The experimental results showed that the determination of linear correlation of these three antimicrobial reagents were above 0.99 with the linear detection range at 10 μg/mL～200 μg/mL.After testing the contents of benzoic acid,sorbic acid and dehydroacetic acid in five kinds of mooncakes by this method,the results demonstrated that all of these samples contained sorbic acid,three of samples contained dehydroacetic acid,and no samples contained benzoic acid.
