CAJ | 학술논문

目的探讨不同海水处理时间对肺泡上皮细胞损伤的影响及肺泡上皮细胞低氧诱导因子1α(HIF-1α)蛋白表达的变化.方法将肺泡上皮细胞来源的A549细胞系分为对照组(C)和海水处理组(S),按海水处理时间不同,S组再分为S1～S6组.光镜观察瑞氏-吉姆萨染色后细胞形态变化,MTT检测细胞增殖,蛋白免疫印记法检测细胞低氧诱导因子1α蛋白的表达.结果 (1)海水处理后,部分细胞出现体积缩小、变圆,核浓缩、深染,核碎裂表现；(2)与对照组相比,海水处理组A549细胞的生长曲线发生变化,海水处理2、4、8h组细胞生长抑制率与对照组比较差异有统计学意义(P＜0.05)；海水处理4h、8h组细胞生长至48 h[(14.25±3.90)％,(27.73±2.09)％]和72 h[(23.13±6.60)％,(39.23±3.78)％]时生长抑制率随海水处理时间延长而增加(P＜0.05)；(3)与对照组相比,HIF-1α在海水处理1h后开始升高,4h达最高峰(P＜0.01),此后略有下降,但仍显著高于对照组(P＜0.01).结论海水长时间处理可抑制肺泡上皮细胞生长,海水处理可诱导肺泡上皮细胞HIF-1α的表达,HIF-1α在海水致肺泡上皮细胞损伤中可能起着重要作用.
목적 탐토불동해수처리시간대폐포상피세포손상적영향급폐포상피세포저양유도인자1α(HIF-1α)단백표체적변화.방법 장폐포상피세포래원적A549세포계분위대조조(C)화해수처리조(S),안해수처리시간불동,S조재분위S1～S6조.광경관찰서씨-길모살염색후세포형태변화,MTT검측세포증식,단백면역인기법검측세포저양유도인자1α단백적표체.결과 (1)해수처리후,부분세포출현체적축소、변원,핵농축、심염,핵쇄렬표현；(2)여대조조상비,해수처리조A549세포적생장곡선발생변화,해수처리2、4、8h조세포생장억제솔여대조조비교차이유통계학의의(P＜0.05)；해수처리4h、8h조세포생장지48 h[(14.25±3.90)％,(27.73±2.09)％]화72 h[(23.13±6.60)％,(39.23±3.78)％]시생장억제솔수해수처리시간연장이증가(P＜0.05)；(3)여대조조상비,HIF-1α재해수처리1h후개시승고,4h체최고봉(P＜0.01),차후략유하강,단잉현저고우대조조(P＜0.01).결론 해수장시간처리가억제폐포상피세포생장,해수처리가유도폐포상피세포HIF-1α적표체,HIF-1α재해수치폐포상피세포손상중가능기착중요작용.
Objective To investigate the damage to alveolar epithelial cells and changes in the expression of hypoxia-inducible factor-lα in alveolar epithelial cells induced by seawater treatment at different time pointes.Methods In accordance with the derivation from alveolar epithelial cells,the A549 cells were divided into the control group (C group) and seawater treatment group (S group).After Wright-Giemsa staining,changes in cell morphology was observed under the light microscope,cell proliferation was detected by MTF (Methylcyclopentadienyl Manganese Tricarbonyl) and expression of hypoxia-inducible factor-1α (HIF-1α) was detected bv Western blot.Results (1) Cell morphology showed that some cells shrank,with a spherical form,nuclear enrichment,hyperchromasia and nuclear fragmentation following seawater treatment.(2) When compared with the control group,changes in the growth curve of A549 cells could be noted in the seawater treatment group.Statistical differences could also be seen in cell growth inhibition rates for the 2 h,4 h,8 h groups,when compared with that of the control group (P ＜ 0.05).(3) When cells grew for 48 h [(14.25±3.90),(27.73 ±2.09)％] and 72 h [(14.25 ±3.90),(27.73 ±2.09)％],the growth inhibition rates of the 4h,8h groups increased with the extension of seawater treatment time.(4)Compared with the control group,the expression of HIF-1 α increased after seawater treatment for 1 h,and reached the peak at 4h (P ＜ 0.01),then decreased slightly,yet still significantly higher than that of the control group (P ＜0.01).Conclusions Prolonged seawater treatment could result in growth inhibition of alveolar epithelial cells,and in the meantime could induce the expression of HIF-1α in alveolar epithelial cells.HIF-1α seemed to play an important role in the damage to alveolar epithelial cells induced by seawater.