中国中医药信息杂志
中國中醫藥信息雜誌
중국중의약신식잡지
CHINESE JOURNAL OF INFORMATION ON TRADITIONAL CHINESE MEDICINE
2014年
5期
78-80
,共3页
玄参配方颗粒%哈巴苷%哈巴俄苷%肉桂酸%高效液相色谱-紫外双波长法
玄參配方顆粒%哈巴苷%哈巴俄苷%肉桂痠%高效液相色譜-紫外雙波長法
현삼배방과립%합파감%합파아감%육계산%고효액상색보-자외쌍파장법
Scrophulariae Radix dispensing particles%harpagide%harpagoside%cinnamic acid%HPLC-UV dual wavelength spectrometry
目的：建立高效液相色谱-紫外双波长法同时测定玄参配方颗粒中3种主要成分哈巴苷、哈巴俄苷和肉桂酸含量的方法。方法采用Ultimate AQ-C18色谱柱(250 mm×4.6 mm,5μm),流动相为1%冰醋酸水溶液和乙腈,梯度洗脱,流速1.0 mL/min,检测波长前13 min为210 nm,13 min后为278 nm,柱温30℃。结果哈巴苷、哈巴俄苷、肉桂酸的线性范围分别为0.06654～0.6654μg(r＝1.0000)、0.02423～0.2423μg (r＝0.9999)、0.10028～1.0028μg(r＝0.9999),平均加样回收率(n＝6)分别为99.80%±1.22%、100.31%±1.30%、100.22%±1.24%。结论本法简单、灵敏度高、重复性好、准确可靠,可为玄参配方颗粒的质量控制以及标准研究提供参考依据。
目的：建立高效液相色譜-紫外雙波長法同時測定玄參配方顆粒中3種主要成分哈巴苷、哈巴俄苷和肉桂痠含量的方法。方法採用Ultimate AQ-C18色譜柱(250 mm×4.6 mm,5μm),流動相為1%冰醋痠水溶液和乙腈,梯度洗脫,流速1.0 mL/min,檢測波長前13 min為210 nm,13 min後為278 nm,柱溫30℃。結果哈巴苷、哈巴俄苷、肉桂痠的線性範圍分彆為0.06654～0.6654μg(r＝1.0000)、0.02423～0.2423μg (r＝0.9999)、0.10028～1.0028μg(r＝0.9999),平均加樣迴收率(n＝6)分彆為99.80%±1.22%、100.31%±1.30%、100.22%±1.24%。結論本法簡單、靈敏度高、重複性好、準確可靠,可為玄參配方顆粒的質量控製以及標準研究提供參攷依據。
목적：건립고효액상색보-자외쌍파장법동시측정현삼배방과립중3충주요성분합파감、합파아감화육계산함량적방법。방법채용Ultimate AQ-C18색보주(250 mm×4.6 mm,5μm),류동상위1%빙작산수용액화을정,제도세탈,류속1.0 mL/min,검측파장전13 min위210 nm,13 min후위278 nm,주온30℃。결과합파감、합파아감、육계산적선성범위분별위0.06654～0.6654μg(r＝1.0000)、0.02423～0.2423μg (r＝0.9999)、0.10028～1.0028μg(r＝0.9999),평균가양회수솔(n＝6)분별위99.80%±1.22%、100.31%±1.30%、100.22%±1.24%。결론본법간단、령민도고、중복성호、준학가고,가위현삼배방과립적질량공제이급표준연구제공삼고의거。
Objective To construct a simultaneous HPLC-UV dual wavelength spectrometry method for detecting three main contents - harpagide, harpagoside and cinnamic acid in Scrophulariae Radix dispensing particles. Methods Ultimate AQ-C18 column (250 mm×4.6 mm, 5μm) was used, with 1%acetic acid solution and acetonitrile as mobile phase of gradient elution. The flow rate was 1.0 mL/min, detection wavelength was 210 nm at former 13 min and 278 nm after 13 min. The column temperature was 30 ℃. Results The linear range of harpagide, harpagoside and cinnamic acid was 0.066 54-0.665 4 μg (r=1.000 0), 0.024 23-0.242 3 μg (r=0.999 9), and 0.100 28-1.002 8 μg (r=0.999 9), respectively. The average recovery (n=6) was 99.80%±1.22%, 100.31%±1.30% and 100.22%±1.24%, respectively. Conclusion The method is simple, repeatable, stable, and can be used for quality control and standardization of Scrophulariae Radix dispensing particles.
