CAJ | 학술논문

目的：建立人皮肤鳞癌细胞体外三维培养模型，分离高侵袭性细胞亚群，并比较其生物学特性。方法利用复方壳多糖组织工程皮肤，成功建立人皮肤鳞癌细胞体外三维培养模型，消化分离得到高侵袭性鳞癌细胞亚群，比较肉桂醛和干扰素作用后对不同侵袭特性细胞亚群的增殖抑制效果，同时比较不同侵袭特性细胞亚群在裸鼠中成瘤效果的不同。结果不同浓度的肉桂醛和干扰素对皮肤鳞癌细胞的增殖抑制差异有统计学意义（P＜0．01），不同侵袭特性的皮肤鳞癌细胞亚群之间的增殖抑制差异也有统计学意义（P＜0．01），另外，两种不同侵袭特性的细胞亚群的成瘤特性差异有统计学意义（P＜0．01）。结论成功建立人皮肤鳞癌体外三维培养模型，同时运用此种三维培养模型能够筛选得到侵袭特性更强的细胞亚群。
목적：건립인피부린암세포체외삼유배양모형，분리고침습성세포아군，병비교기생물학특성。방법이용복방각다당조직공정피부，성공건립인피부린암세포체외삼유배양모형，소화분리득도고침습성린암세포아군，비교육계철화간우소작용후대불동침습특성세포아군적증식억제효과，동시비교불동침습특성세포아군재라서중성류효과적불동。결과불동농도적육계철화간우소대피부린암세포적증식억제차이유통계학의의（P＜0．01），불동침습특성적피부린암세포아군지간적증식억제차이야유통계학의의（P＜0．01），령외，량충불동침습특성적세포아군적성류특성차이유통계학의의（P＜0．01）。결론성공건립인피부린암체외삼유배양모형，동시운용차충삼유배양모형능구사선득도침습특성경강적세포아군。
Objective To establish the three-dimensional culture model of the human skin squamous-celled carcinoma ,and isolate the highly invasive cell subsets ,and compare their biological characteristics .Methods By using the composite chitosan tissue engi-neering skin ,the three-dimensional culture model of the human skin squamous-celled carcinoma was successfully established , through digestion ,we divided and got the high-invasive squamous carcinoma cells subsets .After that ,the different invasive proper-ties of subpopulations of cells in nude mice tumor effects were compared .Results Different concentrations of cinnamaldehyde and interferon on skin squamous-celled carcinoma cell proliferation inhibition had statistical significance (P<0 .01) ,the different invasive characteristics of skin squamous cells carcinoma between subpopulations of cells proliferation inhibition also was statistically signifi-cant(P<0 .01) ,and the two different invasive properties of subpopulations of cells in the tumor characteristics also had statistical significance(P<0 .01) .Conclusion We established the three-dimensional culture model of the human skin squamous-celled carcino-ma successfully ,by using this model ,we could screen the higher invasive cells subsets .