CAJ | 학술논문

目的：检测肾癌组织及癌旁正常组织中miR-16的表达，分析miR-16表达水平与临床病理特征之间的关系，为深入研究miR-16在肾癌的发生发展中功能作用奠定基础。方法在标本库中随机挑选48例手术切除的肾癌及配对癌旁组织标本，提取总RNA并通过反转录获得cDNA，实时荧光定量 PCR（qRT-PCR）检测标本中 miR-16表达量，统计学分析其表达量与患者临床病理特征间的联系。结果48对标本中41例（85.42%）肾癌标本miR-16表达上调，统计学分析证实肾癌组织中miR-16表达量明显高于配对的癌旁正常组织（P＜0.01）。肾癌中miR-16表达量与患者性别、年龄、肾癌组织类型、TNM 分期、AJCC 临床分期无明显相关（P＞0.05）。结论 miR-16在肾癌标本中明显表达上调，提示其可能与肾癌的发生发展有关。
목적：검측신암조직급암방정상조직중miR-16적표체，분석miR-16표체수평여림상병리특정지간적관계，위심입연구miR-16재신암적발생발전중공능작용전정기출。방법재표본고중수궤도선48례수술절제적신암급배대암방조직표본，제취총RNA병통과반전록획득cDNA，실시형광정량 PCR（qRT-PCR）검측표본중 miR-16표체량，통계학분석기표체량여환자림상병리특정간적련계。결과48대표본중41례（85.42%）신암표본miR-16표체상조，통계학분석증실신암조직중miR-16표체량명현고우배대적암방정상조직（P＜0.01）。신암중miR-16표체량여환자성별、년령、신암조직류형、TNM 분기、AJCC 림상분기무명현상관（P＞0.05）。결론 miR-16재신암표본중명현표체상조，제시기가능여신암적발생발전유관。
Objective To detect the expression of miR-16 in renal cell carcinoma (RCC) and investigate the correlations between expression of miR-16 and clinicopathologic characteristics for further study of the role of miR-16 in RCC. Methods Total RNA isolated from 48 RCC tissues and paired adjacent normal tissues resected from nephrectomy was used for obtaining cDNA by performing reverse transcription. The expression of miR-16 was detected by quantitative real-time PCR (qRT-PCR) and the its relationships with clinicopathologic characteristic were explored by statistic analysis. Results The expression of miR-16 in RCC tissues was significantly higher than that in paired adjacent normal tissues (P<0.01), with 41 cases (85.42%) showing up-regulation of miR-16. No relationships were found between the expression of miR-16 and patient's age, gender, histology, TNM stage or AJCC stage. Conclusion miR-16 is up-regulated in RCC, indicating it may be involved in the tumorigenesis and development of RCC.