CAJ | 학술논문

目的：建立薄荷中咖啡酸与蒙花苷的含量测定方法。方法采用HPLC法测定咖啡酸的含量，以Zorbax SB C18为色谱柱；流动相为乙腈-0.1%磷酸溶液（13：87）；检测波长为323nm；流速为1ml·min-1；柱温为30℃。采用HPLC法测定蒙花苷的含量，以Zorbax SB C18为色谱柱；流动相为甲醇-0.1%磷酸溶液（48：52）；检测波长为334nm；流速为1ml·min-1；柱温为30℃。结果咖啡酸在0.0202～0.4040μg范围内线性关系良好（r=0.9999），平均加样回收率为98.0%（RSD=1.16%）。蒙花苷在0.026～0.520μg范围内线性关系良好（r=0.9999），平均加样回收率为99.0%（RSD=1.22%）。结论方法简便、重复性好，为薄荷的质量控制研究提供了一定的参考。
목적：건립박하중가배산여몽화감적함량측정방법。방법채용HPLC법측정가배산적함량，이Zorbax SB C18위색보주；류동상위을정-0.1%린산용액（13：87）；검측파장위323nm；류속위1ml·min-1；주온위30℃。채용HPLC법측정몽화감적함량，이Zorbax SB C18위색보주；류동상위갑순-0.1%린산용액（48：52）；검측파장위334nm；류속위1ml·min-1；주온위30℃。결과가배산재0.0202～0.4040μg범위내선성관계량호（r=0.9999），평균가양회수솔위98.0%（RSD=1.16%）。몽화감재0.026～0.520μg범위내선성관계량호（r=0.9999），평균가양회수솔위99.0%（RSD=1.22%）。결론방법간편、중복성호，위박하적질량공제연구제공료일정적삼고。
Objective To set up the determination method of caffeic acid and linarin content in Herba Menthae. Methods Using HPLC to determine the cafffeic acid.Set chromatography column as Zorbax SB C18 mobile phase as acetonitrile-0.1% phosphoric acid(13:87);detection wavelength was 323nm;flow rate of 1ml·min-1; column temperature was 30℃.Using HPLC to determine the linarin content,set chromatography column as Zorbax SB C18;mobile phase as methanol-0.1% phosphoric acid (48:52);detection wavelength was 334nm;flow rate of 1ml﹒min-1;column temperature was 30℃.Results In the range of 0.0202~0.4040μg,caffeic acid showed good linear relationship(r=0.9999),the average recovery was 98.0%(RSD=1.16%).Linarin in the range0.026~0.520μg also showed good linear relationship(r=0.9999),the average recovery was 99.0%(RSD=1.22%).Conclusion HPLC is a simple,reproducible method which provides valuable reference for the study of Herba Menthae quality control.