CAJ | 학술논문

目的：对柠檬酸及其盐类中嗜酸耐热菌进行培养,研究最佳检测方法。方法通过改变培养基种类、pH 值和热休克方式三个因素,以菌落数多少进行综合评价,优选最佳检测方法。结果检测嗜酸耐热菌的最佳条件：70℃热处理20 min,室温水冷却,用滤膜抽滤后接种于pH值为3.5~4.0的YSG培养基上,置45℃培养5d。结论该方法对柠檬酸及其盐类中嗜酸耐热菌的检测有较强的实用性。
목적：대저몽산급기염류중기산내열균진행배양,연구최가검측방법。방법통과개변배양기충류、pH 치화열휴극방식삼개인소,이균락수다소진행종합평개,우선최가검측방법。결과검측기산내열균적최가조건：70℃열처리20 min,실온수냉각,용려막추려후접충우pH치위3.5~4.0적YSG배양기상,치45℃배양5d。결론해방법대저몽산급기염류중기산내열균적검측유교강적실용성。
Objective To determine the best method to detect thermo-acidophilic bacteria in citric acid and citrate. Methods Three factors (culture medium, pH value and ways of heat shock) were changed, The results were compositive scored according to the number of colonies. Results A better method was as follows:the citric acid solution was heated at 70℃for 20 min, cooled by water of room temperature, filtrated, and then spread on YSG culture medium with a pH value of 3.5~4.0. Sample was incubated at 45℃for 5 days. Conclu-sion This method was a practical method of detecting thermo-acidophilic bacteria in citric acid and citrate.