中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2014年
1期
170-174
,共5页
马泳泳%黄进%周淑娟%葛杭萍%俞康
馬泳泳%黃進%週淑娟%葛杭萍%俞康
마영영%황진%주숙연%갈항평%유강
含IQ模序的GTP酶活化蛋白1%多发性骨髓瘤%姜黄素%细胞迁移%肿瘤侵袭
含IQ模序的GTP酶活化蛋白1%多髮性骨髓瘤%薑黃素%細胞遷移%腫瘤侵襲
함IQ모서적GTP매활화단백1%다발성골수류%강황소%세포천이%종류침습
IQ motif-containing GTPase-activating protein 1%Multiple myeloma%Curcumin%Cell migration%Neoplasm invasiveness
目的:探讨姜黄素对骨髓瘤细胞迁移侵袭能力的影响及其机制.方法:shRNA表达质粒沉默含IQ模序的RasGTP酶活化蛋白1(IQ motif-containing GTPase-activating protein 1,IQGAPI)后转染RPMI8226细胞,Western blotting法检测RPMI8226-shIQGAP1组、RPMI8226-shRNA阴性对照组和未转染RPMI8226组细胞IQGAP1表达;不同浓度姜黄素作用于各组细胞后,Transwell迁移实验和Matrigel侵袭实验检测细胞的迁移及侵袭力,RT-PCR法检测姜黄素作用后IQGAP1 mRNA的表达,Western blotting检测姜黄素对各组细胞IQGAP1蛋白表达的影响.结果:使用shRNA表达质粒沉默IQGAPI后,RPMI8226细胞IQGAP1表达减少;RPMI8226-shIQGAP1组较RP-MI8226-shRNA阴性对照组和未转染RPMI8226组细胞迁移及侵袭力下降,姜黄素可降低RPMI8226-shRNA阴性对照组和未转染RPMI8226组细胞迁移及侵袭力,无浓度相关性,不同浓度的姜黄素对RPMI8226-shIQGAP1组作用后细胞的迁移及侵袭力无明显变化.对于RPMI8226-shRNA阴性对照组及未转染RPMI8226组,姜黄素作用后IQ-GAP1 mRNA及蛋白的表达下降.结论:姜黄素通过抑制IQGAP1的表达降低骨髓瘤细胞的迁移力和侵袭力.
目的:探討薑黃素對骨髓瘤細胞遷移侵襲能力的影響及其機製.方法:shRNA錶達質粒沉默含IQ模序的RasGTP酶活化蛋白1(IQ motif-containing GTPase-activating protein 1,IQGAPI)後轉染RPMI8226細胞,Western blotting法檢測RPMI8226-shIQGAP1組、RPMI8226-shRNA陰性對照組和未轉染RPMI8226組細胞IQGAP1錶達;不同濃度薑黃素作用于各組細胞後,Transwell遷移實驗和Matrigel侵襲實驗檢測細胞的遷移及侵襲力,RT-PCR法檢測薑黃素作用後IQGAP1 mRNA的錶達,Western blotting檢測薑黃素對各組細胞IQGAP1蛋白錶達的影響.結果:使用shRNA錶達質粒沉默IQGAPI後,RPMI8226細胞IQGAP1錶達減少;RPMI8226-shIQGAP1組較RP-MI8226-shRNA陰性對照組和未轉染RPMI8226組細胞遷移及侵襲力下降,薑黃素可降低RPMI8226-shRNA陰性對照組和未轉染RPMI8226組細胞遷移及侵襲力,無濃度相關性,不同濃度的薑黃素對RPMI8226-shIQGAP1組作用後細胞的遷移及侵襲力無明顯變化.對于RPMI8226-shRNA陰性對照組及未轉染RPMI8226組,薑黃素作用後IQ-GAP1 mRNA及蛋白的錶達下降.結論:薑黃素通過抑製IQGAP1的錶達降低骨髓瘤細胞的遷移力和侵襲力.
목적:탐토강황소대골수류세포천이침습능력적영향급기궤제.방법:shRNA표체질립침묵함IQ모서적RasGTP매활화단백1(IQ motif-containing GTPase-activating protein 1,IQGAPI)후전염RPMI8226세포,Western blotting법검측RPMI8226-shIQGAP1조、RPMI8226-shRNA음성대조조화미전염RPMI8226조세포IQGAP1표체;불동농도강황소작용우각조세포후,Transwell천이실험화Matrigel침습실험검측세포적천이급침습력,RT-PCR법검측강황소작용후IQGAP1 mRNA적표체,Western blotting검측강황소대각조세포IQGAP1단백표체적영향.결과:사용shRNA표체질립침묵IQGAPI후,RPMI8226세포IQGAP1표체감소;RPMI8226-shIQGAP1조교RP-MI8226-shRNA음성대조조화미전염RPMI8226조세포천이급침습력하강,강황소가강저RPMI8226-shRNA음성대조조화미전염RPMI8226조세포천이급침습력,무농도상관성,불동농도적강황소대RPMI8226-shIQGAP1조작용후세포적천이급침습력무명현변화.대우RPMI8226-shRNA음성대조조급미전염RPMI8226조,강황소작용후IQ-GAP1 mRNA급단백적표체하강.결론:강황소통과억제IQGAP1적표체강저골수류세포적천이력화침습력.
