CAJ | 학술논문

目的：观察大鼠面神经元缺血后不同时间点硝基化亲环蛋白D（CypD）的表达水平，同时检测缺血大鼠面神经元内线粒体通透性转运孔（MPTP）开放程度，探讨硝基化CypD（N-CypD）在MPTP开放中的作用。方法通过阻断大鼠岩动脉建立面神经元缺血模型；取SD大鼠85只，分为对照组（NC）、假手术组（SH）、岩动脉阻断损伤（PAI）组、PAI+生理盐水（PS）组、PAI+亚精胺（SPD）组。PAI、PAI+PS及PAI+SPD组再分为5个亚组（1d、3d、7d、14d、21d），每组5只大鼠。采用免疫沉淀法（IP）及Western blot法（WB）检测N-CypD表达水平，利用分光光度计检测MPTP的开放程度。结果 IP结果显示，PAI组与PAI+PS组各时间点N-CypD表达水平差异无统计学意义（P>0.05）；PAI+SPD组各时间点N-CypD表达水平较PAI组均降低，差异有统计学意义（P<0.05）；PAI及PAI+PS组各时间点MPTP开放程度差异无统计学意义（P>0.05），PAI+SPD组各时间点MPTP开放程度较PAI组均降低，差异有统计学意义（P<0.05）。相关分析显示，缺血面神经元内N-CypD表达水平与MPTP开放程度呈显著正相关（r=0.783，P<0.01）。结论大鼠面神经元缺血后，神经元内N-CypD表达水平不断增加，降低N-CypD水平可减少MPTP开放，说明N-CypD参与介导损伤神经元中MPTP的开放。
목적：관찰대서면신경원결혈후불동시간점초기화친배단백D（CypD）적표체수평，동시검측결혈대서면신경원내선립체통투성전운공（MPTP）개방정도，탐토초기화CypD（N-CypD）재MPTP개방중적작용。방법통과조단대서암동맥건립면신경원결혈모형；취SD대서85지，분위대조조（NC）、가수술조（SH）、암동맥조단손상（PAI）조、PAI+생리염수（PS）조、PAI+아정알（SPD）조。PAI、PAI+PS급PAI+SPD조재분위5개아조（1d、3d、7d、14d、21d），매조5지대서。채용면역침정법（IP）급Western blot법（WB）검측N-CypD표체수평，이용분광광도계검측MPTP적개방정도。결과 IP결과현시，PAI조여PAI+PS조각시간점N-CypD표체수평차이무통계학의의（P>0.05）；PAI+SPD조각시간점N-CypD표체수평교PAI조균강저，차이유통계학의의（P<0.05）；PAI급PAI+PS조각시간점MPTP개방정도차이무통계학의의（P>0.05），PAI+SPD조각시간점MPTP개방정도교PAI조균강저，차이유통계학의의（P<0.05）。상관분석현시，결혈면신경원내N-CypD표체수평여MPTP개방정도정현저정상관（r=0.783，P<0.01）。결론대서면신경원결혈후，신경원내N-CypD표체수평불단증가，강저N-CypD수평가감소MPTP개방，설명N-CypD삼여개도손상신경원중MPTP적개방。
Objective To explore the expression of nitrated Cyclophilin-D in rat facial nucleus during different stages after interrupting the petrosal artery and its role in the regulation of mitochondrial permeability transition pore opening. Meth-ods The models of facial nucleus ischemia were established in rats by interrupting the petrosal artery. 85 SD rats were divided into 5 groups randomly:NC group,SH group,PAI group,PAI+PS group,PAI+SPD group. NC group and SH group included 5 rats respectively. Other groups were divided into 5 subgroups (1d,3d,7d,14d,21d),each group contained 5 rats. The expressions of nitrated CypD were detected by IP and WB. MPTP opening was measured by spectrophotometer. Results The results of IP showed that the level of nitrated CypD in PAI+SPD group was lower than that in PAI or PAI+PS group,and the difference was significant (P<0.05). Meanwhile the level of the opening MPTP decreased. The correlation analysis showed that the opening of MPTP in facial nucleus was closely associated with the increased expression of N-CypD after interrupting the petrosal artery (r=0.783,P<0.01). Conclusion N-CypD mediate the opening of MPTP, and reducing the expression of the N-CypD would de-crease the level of MPTP opening.