CAJ | 학술논문

目的：建立RP-HPLC法测定人血浆中5-氟尿嘧啶的浓度。方法采用Agilent Eclipse XDB-C18色谱柱，流动相为0.02mol· L -1的磷酸二氢钾缓冲溶液（pH＝3.0），流速为1.0mL· min -1，检测波长为264nm，柱温为25℃。结果5-氟尿嘧啶在2.0～30.0μg· mL -1度范围内呈良好的线性关系，检测限为4.0ng。5-氟尿嘧啶浓度为2.0、10.0、20.0μg· mL -1的日内RSD分别为3.6％、2.2％、1.1％，日间 RSD分别为5.2％、3.4％、1.8％，平均回收率分别为105.3％、97.2％、98.6％。结论该方法简便、快速、准确、重现性好，适用于5-氟尿嘧啶的药代动力学研究及临床血药浓度检测。
목적：건립RP-HPLC법측정인혈장중5-불뇨밀정적농도。방법채용Agilent Eclipse XDB-C18색보주，류동상위0.02mol· L -1적린산이경갑완충용액（pH＝3.0），류속위1.0mL· min -1，검측파장위264nm，주온위25℃。결과5-불뇨밀정재2.0～30.0μg· mL -1도범위내정량호적선성관계，검측한위4.0ng。5-불뇨밀정농도위2.0、10.0、20.0μg· mL -1적일내RSD분별위3.6％、2.2％、1.1％，일간 RSD분별위5.2％、3.4％、1.8％，평균회수솔분별위105.3％、97.2％、98.6％。결론해방법간편、쾌속、준학、중현성호，괄용우5-불뇨밀정적약대동역학연구급림상혈약농도검측。
OBJECTIVE To establish a RP-HPLC method for determining the concentration of 5-fluorouracil in human plasma.METHODS Agilent Eclipse XDB-C18 was adopted with 0.02 mol· L-1 KH2 PO4 buffer solution ( pH=3.0 ) as the mobile phase.The flow rate was 1.0 mL · min -1 , the wavelength was set at 264 nm and the column temperature was 25℃.RESULTS The calibration curve of 5-fluorouracil was linear in the range of 2.0 to 30.0μg · mL-1.The limit of determination was 4.0 ng.The intra-day RSD of 2.0、10.0、20.0μg · mL-1 of 5-fluorouracil were 3.6%、2.2%、1.1%, the inter-day RSD of 2.0、10.0、20.0μg · mL-1 of 5-fluorouracil were 5.2%、3.4%、1.8%,and the average recovery were 105.3%、97.2%、98.6%respectively.CONCLUSION The method is sim-ple,rapid,accurate and reproducible.It is suitable for the pharmacokinetics study and concentration determination of 5-fluorouracil in human plasma.