CAJ | 학술논문

目的：探讨肺炎支原体（MP）咽拭子聚合酶链反应（PCR）法和血清间接免疫荧光法（IIFA）两种检测方法的不同。方法：将198例疑似MP感染的患儿根据年龄分为两组：A组（0~3岁，n=75）、B组（3~12岁， n=123），分别取咽拭子用PCR法测MP-DNA，取血清用IIFA法测MP-IgM，比较两种方法的阳性率。结果：MP-DNA检测阳性率为57.07%，MP-IgM检测阳性率为47.47%，两者比较差异有统计学意义（P<0.01）。A组MP-DNA阳性率48.00%明显高于同组MP-IgM阳性率25.33%，差异有统计学意义（P<0.01）；B组MP-DNA和MP-IgM阳性率分别为62.60%、60.98%比较差异无统计学意义（P>0.05）。结论：PCR与IIFA联合检测可以提高MP感染诊断的准确性。
목적：탐토폐염지원체（MP）인식자취합매련반응（PCR）법화혈청간접면역형광법（IIFA）량충검측방법적불동。방법：장198례의사MP감염적환인근거년령분위량조：A조（0~3세，n=75）、B조（3~12세， n=123），분별취인식자용PCR법측MP-DNA，취혈청용IIFA법측MP-IgM，비교량충방법적양성솔。결과：MP-DNA검측양성솔위57.07%，MP-IgM검측양성솔위47.47%，량자비교차이유통계학의의（P<0.01）。A조MP-DNA양성솔48.00%명현고우동조MP-IgM양성솔25.33%，차이유통계학의의（P<0.01）；B조MP-DNA화MP-IgM양성솔분별위62.60%、60.98%비교차이무통계학의의（P>0.05）。결론：PCR여IIFA연합검측가이제고MP감염진단적준학성。
Objective:To compare the differences between polymerase chain reaction(PCR)and indirect immunofluorescence(IIFA)for detecting the Mycoplasma pneumoniae(MP).Method:A total of 198 children with suspected MP infection were divided into group A(0-3 years old,n=75)and group B(3-12 years old,n=123)according to their ages.Oropharyngeal swabs and blood were collected from the children,MP-DNA was detected by PCR and MP-IgM was detected by IIFA separately.The positive rate of two methods were compared.Result:The positive rate of MP-DNA detection and MP-IgM were 57.07%,47.47%,the difference was statistically significant(P<0.01).In group A the positivity of MP-DNA(48.00%)was significantly higher than the MP-IgM(25.33%),the difference was statistically significant(P<0.01), and there was no significant difference of MP-DNA(62.60%)and MP-IgM(60.98%)in group B(P>0.05).Conclusion:The joint detection of PCR and IIFA can improve MP detecting accuracy.