乳业科学与技术
乳業科學與技術
유업과학여기술
JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY
2012年
6期
23-25
,共3页
王多娇%颜春荣%曲斌%卢剑%徐春祥
王多嬌%顏春榮%麯斌%盧劍%徐春祥
왕다교%안춘영%곡빈%로검%서춘상
婴幼儿乳粉%氯霉素%残留%LC—MS—MS
嬰幼兒乳粉%氯黴素%殘留%LC—MS—MS
영유인유분%록매소%잔류%LC—MS—MS
infant milkpowder%chloramphenicol%residue%LC-MS-MS
建立一种高效液相色谱一串联质谱法快速测定婴幼儿乳粉中氯霉素残留的方法。样品以D5-氯霉素为内标,碱化后,经乙酸乙酯提取、低温离心、氮吹浓缩,流动相复溶,采用高效液相色谱一串联质谱法测定。结果表明:在0.1-5μg/L范围内氯霉素呈良好的线性关系,高、中、低浓度回收率为80%~110%,精密度小于15%,定量限为0.1μg/kg。本方法快速、简便,适于乳粉中氯霉素残留的快速测定。
建立一種高效液相色譜一串聯質譜法快速測定嬰幼兒乳粉中氯黴素殘留的方法。樣品以D5-氯黴素為內標,堿化後,經乙痠乙酯提取、低溫離心、氮吹濃縮,流動相複溶,採用高效液相色譜一串聯質譜法測定。結果錶明:在0.1-5μg/L範圍內氯黴素呈良好的線性關繫,高、中、低濃度迴收率為80%~110%,精密度小于15%,定量限為0.1μg/kg。本方法快速、簡便,適于乳粉中氯黴素殘留的快速測定。
건립일충고효액상색보일천련질보법쾌속측정영유인유분중록매소잔류적방법。양품이D5-록매소위내표,감화후,경을산을지제취、저온리심、담취농축,류동상복용,채용고효액상색보일천련질보법측정。결과표명:재0.1-5μg/L범위내록매소정량호적선성관계,고、중、저농도회수솔위80%~110%,정밀도소우15%,정량한위0.1μg/kg。본방법쾌속、간편,괄우유분중록매소잔류적쾌속측정。
A high performance liquid chromatography tandem mass spectrometric (HPLC-MS-MS) method was developed for rapid determination of chloramphenicol residue in infant milk powder. DS-Chloramphenicol was used as internal standard. Samples were alkalified, extracted with ethyl acetate and centrifuged at a low temperature. Afterwards, the supernatant was concentrated by nitrogen gas blowing and dissolved again in HPLC mobile phase before HPLC-MS- MS analysis. An excellent linear relationship between peak area and chloramphenicol concentration was obtained over the concentration range of 0.1 to 5 μg/L. Average recoveries of chloramphenicol in a blank matrix across three spike levels ranged from 80% to 110%, and inter-batch and intra-batch precision (RSD) was less than 15%. The limit of quantification of this method was 0.1 μg/kg. This method was rapid, simple and suitable for rapid determination of chloramphenicol residue in infant milk powder.