畜牧与饲料科学
畜牧與飼料科學
축목여사료과학
ANIMAL HUSBANDRY AND FEED SCIENCE
2012年
4期
11-13
,共3页
鲁燕%申之义%关平原%希尼尼根
魯燕%申之義%關平原%希尼尼根
로연%신지의%관평원%희니니근
羊传染性脓疱病毒%PCR%克隆%序列分析
羊傳染性膿皰病毒%PCR%剋隆%序列分析
양전염성농포병독%PCR%극륭%서렬분석
contagious ecthyma virus%PCR%cloning%sequence analysis
参考GenBank中羊传染性脓疱NZ2毒株GIF（GM-CSFinhibitory factor）基因序列,设计合成特异性引物,以羊传染性脓疱病毒内蒙古分离毒株（OV/nm-05）为模板,提取总DNA,用PCR技术特异扩增出该毒株GIF基因片段,将其克隆到pEASY-T1载体,鉴定后测序。应用计算机软件将OV/nm-05基因测序结果与参考毒株OV-NZ2、OV-D1701、OV-IA82、OV-SA00、OV-F07.808R、OV-MUK59/05、OV-F94.848R的GIF基因序列进行比较。结果表明,OV/nm-05与7株参考毒株的同源性分别为95.7%、98.2%、96.7%、95.5%、95.9%、95.5%、95.1%。
參攷GenBank中羊傳染性膿皰NZ2毒株GIF（GM-CSFinhibitory factor）基因序列,設計閤成特異性引物,以羊傳染性膿皰病毒內矇古分離毒株（OV/nm-05）為模闆,提取總DNA,用PCR技術特異擴增齣該毒株GIF基因片段,將其剋隆到pEASY-T1載體,鑒定後測序。應用計算機軟件將OV/nm-05基因測序結果與參攷毒株OV-NZ2、OV-D1701、OV-IA82、OV-SA00、OV-F07.808R、OV-MUK59/05、OV-F94.848R的GIF基因序列進行比較。結果錶明,OV/nm-05與7株參攷毒株的同源性分彆為95.7%、98.2%、96.7%、95.5%、95.9%、95.5%、95.1%。
삼고GenBank중양전염성농포NZ2독주GIF（GM-CSFinhibitory factor）기인서렬,설계합성특이성인물,이양전염성농포병독내몽고분리독주（OV/nm-05）위모판,제취총DNA,용PCR기술특이확증출해독주GIF기인편단,장기극륭도pEASY-T1재체,감정후측서。응용계산궤연건장OV/nm-05기인측서결과여삼고독주OV-NZ2、OV-D1701、OV-IA82、OV-SA00、OV-F07.808R、OV-MUK59/05、OV-F94.848R적GIF기인서렬진행비교。결과표명,OV/nm-05여7주삼고독주적동원성분별위95.7%、98.2%、96.7%、95.5%、95.9%、95.5%、95.1%。
A pair specific primer were designed and synthesized according to the published GIF（GM-CSF inhibitory factor） sequence of NZ2 strain in GenBank.Total DNA of the contagious ecthyma virus（CEV） strain（OV/nm-05） which was isolated in Inner Mongolia was extracted,and the GIF gene was amplificated.The GIF gene was cloned into pEASY-T1 plasmids and then sequenced.The acquired GIF gene sequences of OV/nm-05 was compared with those of 7 reference strains like OV-NZ2,OV-D1701,OV-IA82,OV-SA00,OV-F07.808R,OV-MUK59/05 and OV-F94.848R.Results showed that the GIF gene homology of OV/nm-05 with 7 reference strains was 95.7%,98.2%,96.7%,95.5%,95.9%,95.5% and 95.1%,respectively.
