CAJ | 학술논문

DGAT1基因作为影响奶牛产奶和肉牛肉质性状的主效基因之一，引起了人们越来越多的关注。本文应用生物信息学方法比较了人、牛、猪、绵羊等24个物种的41DGAT1基因编码区（CDS）核苷酸和蛋白质序列，并对该基因的遗传多样性进行了分析。结果表明，DGAT1基因的CDS核苷酸和蛋白质序列长度多样性丰富；具有终止密码子偏爱性，包括牛在内的所有脊椎动物终止密码子都为TGA；在长度为1187bp的33条基因序列中检测到992个多态位点，共生成26种单倍型。从聚类分析图可以看出，脊椎动物、无脊椎动物、植物和微生物的ODCATl基因之间存在着进化分歧，但界内差异较小。
DGAT1기인작위영향내우산내화육우육질성상적주효기인지일，인기료인문월래월다적관주。본문응용생물신식학방법비교료인、우、저、면양등24개물충적41DGAT1기인편마구（CDS）핵감산화단백질서렬，병대해기인적유전다양성진행료분석。결과표명，DGAT1기인적CDS핵감산화단백질서렬장도다양성봉부；구유종지밀마자편애성，포괄우재내적소유척추동물종지밀마자도위TGA；재장도위1187bp적33조기인서렬중검측도992개다태위점，공생성26충단배형。종취류분석도가이간출，척추동물、무척추동물、식물화미생물적ODCATl기인지간존재착진화분기，단계내차이교소。
In this paper, we used bioinformatics software to analyze the CDS nucleotide and protein sequences of DGAT1 gene of 24 species including Homo sapiens, Bos Taurus, Ovis aries, Sus scrofa and so on. Meawhile, we also analyzed the genetic diversity ofDGAT1 gene. The results indicated that CDS and protein sequences had plentiful length diversity, DGAT1 gene had TGA stop codon bias, but codon bias was not very significant. A total of 992 polymorphic sites and 26 haplotypes in the 1187bp sequence of 33 DGAT1 genes were found in this study. The phylogenetic tree showed that, for DGAT1 gene, there was an evolutionary divergence existed between vertebrate animals, invertebrates animals, plants and microorganisms, but the evolutionary divergence was not sigifinant within kingdom.