CAJ | 학술논문

　　利用农杆菌介导法将抗草甘膦基因(ssu-G2-aroA)转入优良玉米自交系R18-599和齐319，侵染后的愈伤组织转至潮霉素浓度为10 mg/L的筛选培养基上，继代筛选3轮，每轮3周；得到的抗性愈伤组织在含有潮霉素浓度为3 mg/L的分化培养基上进行分化，R18-599获得30株再生植株，齐319获得18株再生植株。经PCR鉴定后共得到7株转化抗性植株(4株R18-599、3株齐319)。经PCR-Southern及RT-PCR检测结果表明，ssu-G2-aroA基因已稳定整合到了玉米基因组中，并在RNA水平上得到表达。
　　이용농간균개도법장항초감련기인(ssu-G2-aroA)전입우량옥미자교계R18-599화제319，침염후적유상조직전지조매소농도위10 mg/L적사선배양기상，계대사선3륜，매륜3주；득도적항성유상조직재함유조매소농도위3 mg/L적분화배양기상진행분화，R18-599획득30주재생식주，제319획득18주재생식주。경PCR감정후공득도7주전화항성식주(4주R18-599、3주제319)。경PCR-Southern급RT-PCR검측결과표명，ssu-G2-aroA기인이은정정합도료옥미기인조중，병재RNA수평상득도표체。
The ssu-G2-aroA gene was transformed into maize inbred lines R18-599 and Qi319 by using A-grobacterium-mediated method. The transformed calli was transferred to the selecting medium with 10 mg/L hy-gromycin for 9 weeks. Thirty plants of R18-599 and 18 plants of Qi319 were obtained from resistant calli which had been cultured on differentiation medium with 3 mg/L hygromycin. Seven transformants(4 plants R18-599, 3 plants Qi319) were obtained by analyses of PCR, and analyses of PCR-southern and RT-PCR on transformants showed that foreign ssu-G2-aroA gene is integrated into maize genome and is over expressed. The obtained transgenic plants bar boring ssu-G2-aroA.