CAJ | 학술논문

将超临界流体萃取与纤维素酶法相结合,对落叶松树皮中原花青素的提取工艺进行了研究。首先,采用超临界CO2从落叶松树皮中提取脂溶性成分,采用萃取压力35 MPa、温度50℃,萃取130 min后,树脂平均得率为2.16%,然后将萃余物通过纤维素酶辅助提取原花青素,通过单因素和正交试验确定了原花青素提取的最佳工艺条件为：3 g脱脂树皮粉以缓冲液为溶剂,原料粒度为150~178μm,纤维酶加入量为原料质量的0.6%,液料比14：1(mL：g),酶解pH值5,酶解温度40℃,酶解时间2 h后,原花青素的得率可以达到7.36%。通过扫描电子显微镜观察和傅立叶变换红外光谱分析表明,提取树脂后的树皮纤维结构有不同程度的破坏,从而促使胞内物质溶出,提高了得率,所获得的落叶松树皮原花青素分子中主要存在原花青定型结构单元,没有使有效成分的结构发生改变。
장초림계류체췌취여섬유소매법상결합,대락협송수피중원화청소적제취공예진행료연구。수선,채용초림계CO2종락협송수피중제취지용성성분,채용췌취압력35 MPa、온도50℃,췌취130 min후,수지평균득솔위2.16%,연후장췌여물통과섬유소매보조제취원화청소,통과단인소화정교시험학정료원화청소제취적최가공예조건위：3 g탈지수피분이완충액위용제,원료립도위150~178μm,섬유매가입량위원료질량적0.6%,액료비14：1(mL：g),매해pH치5,매해온도40℃,매해시간2 h후,원화청소적득솔가이체도7.36%。통과소묘전자현미경관찰화부립협변환홍외광보분석표명,제취수지후적수피섬유결구유불동정도적파배,종이촉사포내물질용출,제고료득솔,소획득적락협송수피원화청소분자중주요존재원화청정형결구단원,몰유사유효성분적결구발생개변。
The supercritical fluid extraction combined with enzymatic hydrolysis technology for extracting proanthocyanidins from the larch bark was studied. Firstly, lipophilic components from the larch bark were extracted by supercritical CO2 at 50 ℃ for 130 min (extraction pressure was 35 MPa), and the average extraction yield of grease was 2. 16%. Then proanthocyanidins were extracted from the raffinate with cellulase-assisted. The optimum extraction condition, through the single factor and orthogonal test to determine were as follows:3 g degreased bark powder, Buffer solution as the solvent, the material for the granularity of the raw material 150-178 μm, 0. 6 % of raw material quality as the amount of the fiber enzyme addition, the liquid-solid ratio 14:1 (mL: g), the pH of enzymatic hydrolysis 5, the enzymolysis temperature 40 ℃. After enzymolysis 2 h, the yield of proanthocyanidins can reach 7. 36 %. The analysis showed that the fiber structure of bark had been damaged with different degrees after the extraction with cellulase-assisted methods by SEM and FT-IR. And hence this facilitated mass transfer and promoted extraction efficiency. The structure unit of procyanidins type is confirmed mainly in the proanthocyanidins molecule of the larch bark. The structure of efficient component was not changed.